Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Musashi 1 / Msi1 antibody [EP1302]

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Adult rat,svz region of lateral ventricle)
Specification
Adult rat,svz region of lateral ventricle
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C

Other product details

Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s) · Diluent: TBS/BSA/Azide

Secondary antibody

Name
Non-Abcam antibody was used: anti rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/300

Additional data

Additional Notes
This is the only Ab reagent that, for me, gives consistent, appropriate immunocalisation of progenitor neurones in the svz of rat lateral ventricle in pwax sections.
Musashi -1 is, however, positive in other areas of developing rat CNS.
Abcam response
Many thanks for your feedback for ab52865 (Musashi 1 / Msi1 antibody [EP1302]) in rat brain tissue on IHC-P.

Mr. Carl Hobbs

Verified customer

Submitted Oct 23 2007

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