Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Musashi 1 / Msi1 antibody [EP1302]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Chicken Tissue sections (developing brain Embyronic day 7)
Specification
developing brain Embyronic day 7
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C

Other product details

Dilution
1/500
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS/BSA/Azide

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
I originally used ab52865 at a dilution factor of 1/200. However, I am now testing this antibody at 1/500. This now seems to be the optimal dilution factor for me.
I recommend a range of 1/200-500 of this lot of ab52865.

Mr. Carl Hobbs

Verified customer

Submitted Nov 05 2007

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