Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Musashi 1 / Msi1 antibody [EP1302]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Quail Tissue sections (embryo d5/6 Brain stem T/S)
Specification
embryo d5/6 Brain stem T/S
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C

Other product details

Dilution
1/300
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS/BSA/Azide

Secondary antibody

Name
Non-Abcam antibody was used: goat anti rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/300

Additional data

Additional Notes
Ab52865 is an excellent reagent for p.wax sections. I have not yet been able to find any other anti Mus-1 Ab that gives such good results. Image shows ventral region of developing quail brain stem.

Mr. Carl Hobbs

Verified customer

Submitted Dec 03 2007

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