Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1302] to Musashi 1 / Msi1 (PE)
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
Product nameAnti-Musashi 1 / Msi1 antibody [EP1302] (PE)
See all Musashi 1 / Msi1 primary antibodies
DescriptionRabbit monoclonal [EP1302] to Musashi 1 / Msi1 (PE)
ConjugationPE. Ex: 488nm, Em: 575nm
SpecificitySeveral customers have found that this antibody gives good results in mouse and rat however in our hands, we cannot obtain positive results. This antibody is therefore no longer covered by our Abpromise guarantee for use in mouse or rat.
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse
Predicted to work with: Chicken, Human, Quail
- Flow Cyt: Neuro 2A (differentiated).
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab210418 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionRNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
Tissue specificityDetected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.
Sequence similaritiesBelongs to the Musashi family.
Contains 2 RRM (RNA recognition motif) domains.
DomainThe first RNA recognition motif binds more strongly to RNA compared to the second one.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- Msi 1 antibody
- Msi1 antibody
- MSI1H_HUMAN antibody
Overlay histogram showing Neuro 2A (differentiated) cells stained with ab210418 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol for 30 min at -20°C. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab210418, 1/2500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
ab210418 has not yet been referenced specifically in any publications.