Several customers have found that this antibody gives good results in rat however in our hands, we cannot obtain positive results. This antibody is therefore no longer covered by our Abpromise guarantee for use in rat.
ICC/IF, WBmore details Unsuitable for:
IHC-FoFr or IHC-P
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Musashi 1/ Msi1.
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.
Belongs to the Musashi family. Contains 2 RRM (RNA recognition motif) domains.
The first RNA recognition motif binds more strongly to RNA compared to the second one.
Western blot - Anti-Musashi 1 / Msi1 antibody (ab21628)
All lanes : Anti-Musashi 1 / Msi1 antibody (ab21628) at 1 µg/ml
Lane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate Lane 2 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205) Lane 3 : E14 Mouse Embryo Brain Tissue Lysate Lane 4 : E10 Mouse Embryo Brain Tissue Lysate Lane 5 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate with Human Musashi 1 / Msi1 peptide (ab23870) at 1 µg/ml Lane 6 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205) with Human Musashi 1 / Msi1 peptide (ab23870) at 1 µg/ml Lane 7 : E14 Mouse Embryo Brain Tissue Lysate with Human Musashi 1 / Msi1 peptide (ab23870) at 1 µg/ml Lane 8 : E10 Mouse Embryo Brain Tissue Lysate with Human Musashi 1 / Msi1 peptide (ab23870) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 39 kDa Observed band size: 39 kDa Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.
ab21628 recognized a band corresponding to the expected size of Musashi1/Msi1. The band was blocked using the immunising peptide (ab23870).
Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody (ab21628)This image is courtesy of an Abreview submitted by Miss Szu-Yu Chen
ab21628 at 1ug/ml staining human eye (limbal region) by ICC/IF. The cells were paraformaldehyde fixed and blocked with 2% BSA prior to incubation with antibody for 1 hour. A FITC conjugated sheep polyclonal antibody was used as the secondary.
ICC/IF image of ab21628 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21628, 10µg/ml) overnight at +4°C. The secondary antibody (green) was a96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) PC12 cells at 10µg/ml.