Product nameAnti-Musashi 1 / Msi1 antibody [EP1302]
See all Musashi 1 / Msi1 primary antibodies
DescriptionRabbit monoclonal [EP1302] to Musashi 1 / Msi1
SpecificitySeveral customers have found that this antibody gives good results in mouse and rat however in our hands, we cannot obtain positive results. This antibody is therefore no longer covered by our Abpromise guarantee for use in mouse or rat.
Tested applicationsSuitable for: IHC-P, ICC/IF, IHC-Fr, WB, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Chicken, Human, Quail
Synthetic peptide within Human Musashi 1/ Msi1 (N terminal). The exact sequence is proprietary.
(Peptide available as
- PC12 cells, SH-SY-5Y cell lysate and human brain tissue.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
- Anti-Musashi 1 / Msi1 antibody [EP1302] (HRP) (ab199556)
- Anti-Musashi 1 / Msi1 antibody [EP1302] (Alexa Fluor® 488) (ab199781)
- Anti-Musashi 1 / Msi1 antibody [EP1302] (Alexa Fluor® 647) (ab199838)
- Anti-Musashi 1 / Msi1 antibody [EP1302] (Phycoerythrin) (ab210418)
- Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab52865 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/250 - 1/500.|
|WB||1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).Can be blocked with Musashi 1 / Msi1 peptide (ab178003).
For unpurified use at 1/2000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
For unpurified use at 1/40.
FunctionRNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
Tissue specificityDetected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.
Sequence similaritiesBelongs to the Musashi family.
Contains 2 RRM (RNA recognition motif) domains.
DomainThe first RNA recognition motif binds more strongly to RNA compared to the second one.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- Msi 1 antibody
- Msi1 antibody
- MSI1H_HUMAN antibody
Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Mushashi 1/ Msi1 with purified ab52865 at 1:80 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Mushashi 1/ Msi1 with Purified ab52865 at 1:500 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200. Ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling Mushashi 1/ Msi1 with Purified ab52865 at 1:50 dilution (17.7 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.
All lanes : Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865) at 1/1000 dilution (purified)
Lane 1 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates
Lane 2 : UMNSAH/DF-1 (chicken embryo fibroblast) whole cell lysates
Lysates/proteins at 20 µg per lane.
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 39 kDa
Blocking and diluting buffer: 5% NFDM/TBST.
Immunocytochemistry/Immunofluorescence analysis of Neuro-2a (mouse neuroblastoma) labelling Musashi 1 with purified ab52865 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilised by 0.1% tritonX-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865) at 1/2000 dilution (unpurified) + SH-SY-5Y cell lysate at 10 µg/ml
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
Immunohistochemistical detection (on formaldehyde/PFA-fixed paraffin-embedded sections) of Musashi 1 / Msi1 antibody [EP1302] (unpurified ab52865) on Quail Tissue sections (embryo d5/6 Brain stem T/S). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Primary Antibody unpurified ab52865 incubated at 1/300 for 2 hours at RT. Secondary Antibody: Biotin labelled goat anti rabbit IgG (1/300).
Flow Cyt image of Musashi1 (ab52865)using Accutase digested single cell suspension of hESC. the cells were fixed adn permeabilized. The cells were incubated with unpurified ab52865 (1/20 using Prem/wash solution) for 30 mins at 23°C.
This product has been referenced in:
- Shou Z et al. Overexpression of Musashi-1 protein is associated with progression and poor prognosis of gastric cancer. Oncol Lett 13:3556-3566 (2017). Read more (PubMed: 28521458) »
- Lu X et al. High-frequency repetitive transcranial magnetic stimulation for treating moderate traumatic brain injury in rats: A pilot study. Exp Ther Med 13:2247-2254 (2017). Read more (PubMed: 28565833) »