Overview

  • Product name

    Anti-Musashi 1 / Msi1 antibody [Y2709]
    See all Musashi 1 / Msi1 primary antibodies
  • Description

    Mouse monoclonal [Y2709] to Musashi 1 / Msi1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    corresponding to Musashi 1/ Msi1.

  • Positive control

    • This antibody gave a positive signal in the following lysates: human brain membrane; E14 rat embryonic brain; SHSY5Y whole cell; 46C whole cell.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab129819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).
IP Use at an assay dependent concentration. (Pubmed ID 20696379)

Target

  • Function

    RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
  • Tissue specificity

    Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.
  • Sequence similarities

    Belongs to the Musashi family.
    Contains 2 RRM (RNA recognition motif) domains.
  • Domain

    The first RNA recognition motif binds more strongly to RNA compared to the second one.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Msi 1 antibody
    • Msi1 antibody
    • MSI1H_HUMAN antibody
    • Musashi homolog 1 antibody
    • Musashi RNA binding protein 1 antibody
    • Musashi-1 antibody
    • Musashi1 antibody
    • RNA binding protein Musashi homolog 1 antibody
    • RNA-binding protein Musashi homolog 1 antibody
    see all

Images

  • All lanes : Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819) at 5 µg/ml

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : MSI1 knockout HAP1 whole cell lysate
    Lane 3 : SH-SY5Y whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 39 kDa
    Observed band size: 39 kDa



    Lanes 1 - 3: Merged signal (red and green). Green - ab129819 observed at 39 kDa. Red - loading control, ab52866, observed at 50 kDa.

    ab129819 was shown to specifically react with Musashi 1 / Msi1 in wild-type HAP1 cells as signal was lost in MSI1 knockout cells. Wild-type and MSI1 knockout samples were subjected to SDS-PAGE. Ab129819 and ab52866 (Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at 5 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819) at 5 µg/ml

    Lane 1 : Brain (Human) Membrane Lysate - fetal normal tissue (ab29457)
    Lane 2 : E14 Rat Embryo Brain Tissue Lysate
    Lane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 4 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 39 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?


    Exposure time: 12 minutes

References

This product has been referenced in:

  • Kuwako K  et al. Neural RNA-binding protein Musashi1 controls midline crossing of precerebellar neurons through posttranscriptional regulation of Robo3/Rig-1 expression. Neuron 67:407-21 (2010). IP . Read more (PubMed: 20696379) »
See 1 Publication for this product

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