Key features and details
- Goat polyclonal to Muscarinic Acetylcholine Receptor 1/CHRM1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Muscarinic Acetylcholine Receptor 1/CHRM1 antibody
See all Muscarinic Acetylcholine Receptor 1/CHRM1 primary antibodies
DescriptionGoat polyclonal to Muscarinic Acetylcholine Receptor 1/CHRM1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Dog, Pig, Rhesus monkey, Orangutan
- Human brain lysate.
This product was previously labelled as Muscarinic Acetylcholine Receptor 1
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab77098 is purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab77098 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 51 kDa.
1 hour primary incubation is recommended for this product.
RelevanceThe muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. Muscarinic receptors are so called as they are more sensitive to the effects of the alkaloid muscarine, rather than nicotine.
Cellular localizationCell Membrane; multi-pass membrane protein
- AChR antibody
- Cholinergic receptor muscarinic 1 antibody
- CHRM1 antibody
Anti-Muscarinic Acetylcholine Receptor 1/CHRM1 antibody (ab77098) at 0.300000011920929 µg/ml + human brain lysate (in RIPA buffer) at 35 µg
Predicted band size: 51 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
ab77098 has been referenced in 1 publication.
- Winder M et al. Autonomic Receptor-mediated Regulation of Production and Release of Nitric Oxide in Normal and Malignant Human Urothelial Cells. Basic Clin Pharmacol Toxicol 121:257-265 (2017). PubMed: 28437032