Product nameAnti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1]
See all Muscarinic Acetylcholine Receptor 2/CM2 primary antibodies
DescriptionMouse monoclonal [31-1D1] to Muscarinic Acetylcholine Receptor 2/CM2
SpecificityThis antibody is specific for the m2 mAChR subtype.
Tested applicationsSuitable for: WB, Flow Cyt, ICC/IF, ELISA, IHC-P, IPmore details
Species reactivityReacts with: Mouse, Rat, Human, Pig
Does not react with: Chicken
Full length native protein (purified) corresponding to Pig Muscarinic Acetylcholine Receptor 2/CM2. Purified from Pig Heart.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 0.1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab2805 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 52 kDa).|
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use a concentration of 5 µg/ml. PubMed: 19889856|
|ELISA||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
FunctionThe muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.
Involvement in diseaseGenetic variations in CHRM2 can influence susceptibility to major depressive disorder (MDD) [MIM:608516]. MDD is one of the most common psychiatric disorders. MDD is a complex trait characterized by one or more major depressive episodes without a history of manic, mixed, or hypomanic episodes. A major depressive episode is characterized by at least 2 weeks during which there is a new onset or clear worsening of either depressed mood or loss of interest or pleasure in nearly all activities. Four additional symptoms must also be present including changes in appetite, weight, sleep, and psychomotor activity; decreased energy; feelings of worthlessness or guilt; difficulty thinking, concentrating, or making decisions; or recurrent thoughts of death or suicidal ideation, plans, or attempts. The episode must be accompanied by distress or impairment in social, occupational, or other important areas of functioning.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily.
Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
- Information by UniProt
- 7TM receptor antibody
- Acetylcholine receptor, muscarinic, 2 antibody
- AChR antibody
All lanes : Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1] (ab2805) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human spinal cord tissue lysate - total protein (ab29188)
Lane 3 : Brain (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted?
Additional bands at: 22 kDa, 40 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Muscarinic Acetylcholine Receptor 2/CM2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Immunohistochemistry was performed on normal biopsies of deparaffinized human kidney tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer and microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a Mouse monoclonal antibody recognizing Muscarinic Acetylcholine Receptor 2/CM2 (ab2805) or without primary antibody (negative control) overnight at 4�C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
ICC/IF image of ab2805 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2805, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Ma G et al. Association of Autoantibodies against M2-Muscarinic Acetylcholine Receptor with Atrial Fibrosis in Atrial Fibrillation Patients. Cardiol Res Pract 2019:8271871 (2019). Read more (PubMed: 30863630) »
- Benoit A et al. Flow cytometry for receptor analysis from ex-vivo brain tissue in adult rat. J Neurosci Methods 304:11-23 (2018). Flow Cyt . Read more (PubMed: 29660368) »