Recombinant Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] (ab109226)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4568] to Muscarinic Acetylcholine Receptor 2/CM2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568]
See all Muscarinic Acetylcholine Receptor 2/CM2 primary antibodies -
Description
Rabbit monoclonal [EPR4568] to Muscarinic Acetylcholine Receptor 2/CM2 -
Host species
Rabbit -
Specificity
Please note: blast analysis of the immunogen sequence for ab109226 shows 64% homology with the Muscarinic Acetylcholine Receptor 3, therefore some reactivity with this receptor subtype may be possible. -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Muscarinic Acetylcholine Receptor 2/CM2 aa 150-250. The exact sequence is proprietary.
Database link: P08172 -
Positive control
- WB: SH-SY5Y, fetal brain, U87-MG, Neuro-2a, and Y79 lysates IHC-P: Human brain tissue IF: SH-SY5Y cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Frozen Stock (-20C). Shelf life 12 months. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4568 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab109226 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). | |
IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. -
Involvement in disease
Genetic variations in CHRM2 can influence susceptibility to major depressive disorder (MDD) [MIM:608516]. MDD is one of the most common psychiatric disorders. MDD is a complex trait characterized by one or more major depressive episodes without a history of manic, mixed, or hypomanic episodes. A major depressive episode is characterized by at least 2 weeks during which there is a new onset or clear worsening of either depressed mood or loss of interest or pleasure in nearly all activities. Four additional symptoms must also be present including changes in appetite, weight, sleep, and psychomotor activity; decreased energy; feelings of worthlessness or guilt; difficulty thinking, concentrating, or making decisions; or recurrent thoughts of death or suicidal ideation, plans, or attempts. The episode must be accompanied by distress or impairment in social, occupational, or other important areas of functioning. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1129 Human
- Entrez Gene: 243764 Mouse
- Entrez Gene: 81645 Rat
- Omim: 118493 Human
- SwissProt: P08172 Human
- SwissProt: Q9ERZ4 Mouse
- SwissProt: P10980 Rat
- Unigene: 535891 Human
see all -
Alternative names
- 7TM receptor antibody
- Acetylcholine receptor, muscarinic, 2 antibody
- AChR antibody
see all
Images
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All lanes : Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] (ab109226) at 1/1000 dilution
Lane 1 : SH-SY5Y lysate
Lane 2 : Fetal brain lysate
Lane 3 : U87-MG lysate
Lane 4 : Neuro-2a lysate
Lane 5 : Y79 lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] (ab109226)
Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab109226 at 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
References (7)
ab109226 has been referenced in 7 publications.
- Bachmann SB et al. A Distinct Role of the Autonomic Nervous System in Modulating the Function of Lymphatic Vessels under Physiological and Tumor-Draining Conditions. Cell Rep 27:3305-3314.e13 (2019). PubMed: 31189113
- Kashyap MP et al. BDNF overexpression in the bladder induces neuronal changes to mediate bladder overactivity. Am J Physiol Renal Physiol 315:F45-F56 (2018). PubMed: 29092846
- O'Hern PJ et al. Decreased microRNA levels lead to deleterious increases in neuronal M2 muscarinic receptors in Spinal Muscular Atrophy models. Elife 6:N/A (2017). WB ; Mouse . PubMed: 28463115
- Zhang L et al. Stress induces more serious barrier dysfunction in follicle-associated epithelium than villus epithelium involving mast cells and protease-activated receptor-2. Sci Rep 7:4950 (2017). PubMed: 28694438
- Zhao Z et al. Involvement of HCN Channel in Muscarinic Inhibitory Action on Tonic Firing of Dorsolateral Striatal Cholinergic Interneurons. Front Cell Neurosci 10:71 (2016). IHC . PubMed: 27047336
- Ferrier J et al. Cholinergic Neurotransmission in the Posterior Insular Cortex Is Altered in Preclinical Models of Neuropathic Pain: Key Role of Muscarinic M2 Receptors in Donepezil-Induced Antinociception. J Neurosci 35:16418-30 (2015). PubMed: 26674867
- Wan D et al. Catalpol Induces Neuroprotection and Prevents Memory Dysfunction through the Cholinergic System and BDNF. Evid Based Complement Alternat Med 2013:134852 (2013). WB . PubMed: 24194776