Product nameAnti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568]
See all Muscarinic Acetylcholine Receptor 2/CM2 primary antibodies
DescriptionRabbit monoclonal [EPR4568] to Muscarinic Acetylcholine Receptor 2/CM2
SpecificityPlease note: blast analysis of the immunogen sequence for ab109226 shows 64% homology with the Muscarinic Acetylcholine Receptor 3, therefore some reactivity with this receptor subtype may be possible.
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Muscarinic Acetylcholine Receptor 2/CM2 aa 150-250. The exact sequence is proprietary.
Database link: P08172
- WB: SH-SY5Y, fetal brain, U87-MG, Neuro-2a, and Y79 lysates IHC-P: Human brain tissue IF: SH-SY5Y cells
Storage instructionsFrozen Stock (-20C). Shelf life 12 months.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109226 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).|
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionThe muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.
Involvement in diseaseGenetic variations in CHRM2 can influence susceptibility to major depressive disorder (MDD) [MIM:608516]. MDD is one of the most common psychiatric disorders. MDD is a complex trait characterized by one or more major depressive episodes without a history of manic, mixed, or hypomanic episodes. A major depressive episode is characterized by at least 2 weeks during which there is a new onset or clear worsening of either depressed mood or loss of interest or pleasure in nearly all activities. Four additional symptoms must also be present including changes in appetite, weight, sleep, and psychomotor activity; decreased energy; feelings of worthlessness or guilt; difficulty thinking, concentrating, or making decisions; or recurrent thoughts of death or suicidal ideation, plans, or attempts. The episode must be accompanied by distress or impairment in social, occupational, or other important areas of functioning.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily.
Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
- Information by UniProt
- 7TM receptor antibody
- Acetylcholine receptor, muscarinic, 2 antibody
- AChR antibody
All lanes : Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] (ab109226) at 1/1000 dilution
Lane 1 : SH-SY5Y lysate
Lane 2 : Fetal brain lysate
Lane 3 : U87-MG lysate
Lane 4 : Neuro-2a lysate
Lane 5 : Y79 lysates
Lysates/proteins at 10 µg per lane.
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab109226 at 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This product has been referenced in:
- Bachmann SB et al. A Distinct Role of the Autonomic Nervous System in Modulating the Function of Lymphatic Vessels under Physiological and Tumor-Draining Conditions. Cell Rep 27:3305-3314.e13 (2019). Read more (PubMed: 31189113) »
- Kashyap MP et al. BDNF overexpression in the bladder induces neuronal changes to mediate bladder overactivity. Am J Physiol Renal Physiol 315:F45-F56 (2018). Read more (PubMed: 29092846) »