Product nameAnti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody
See all Muscarinic Acetylcholine Receptor M3/CHRM3 primary antibodies
DescriptionRabbit polyclonal to Muscarinic Acetylcholine Receptor M3/CHRM3
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig
Recombinant fragment corresponding to Human Muscarinic Acetylcholine Receptor M3/CHRM3 aa 286-461.
Database link: P20309
- WB: NT2D1 whole cell lysate, mouse intestine tissue lysate. IF/ICC: A431 cells, SKNSH cells.
This product was previously labelled as Muscarinic Acetylcholine Receptor M3
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab126168 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 66 kDa.|
|ICC/IF||1/100 - 1/1000.
Fix cells in ice-cold methanol.
FunctionThe muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily.
Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane. Basolateral cell membrane.
- Information by UniProt
- AChR antibody
- ACM3_HUMAN antibody
- cholinergic receptor muscarinic 3 antibody
Immunocytochemistry/ Immunofluorescence analysis of A431 cells labeling Muscarinic Acetylcholine Receptor M3/CHRM3 with ab126168 at 1/500 (green). Cells were fixed in ice-cold MeOH for 5 minutes. Cells were co-stained with Hoechst 33342 staining (blue). Blocking: 2.5% BSA/PBS for 30 min. The primary antibody was incubated for 4°C overnight. The secondary antibody was Rabbit IgG antibody (Alexa Fluor 488), 1/2000 (keep from light), room temperature for 1 hour. Washing using PBS 3 x 3 minutes.
Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (ab126168) at 1/1000 dilution + NT2D1 whole cell extracts at 30 µg
HRP-conjugated anti-rabbit IgG
Predicted band size: 66 kDa
7.5% SDS PAGE
Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (ab126168) at 1/1000 dilution + Mouse intestine tissue lysate at 14 µg
HRP-conjugated goat anti-Rabbit IgG (H+L) polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 66 kDa
Additional bands at: 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 seconds
ICC/IF image of ab126168 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab126158, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Mona M et al. MIST1, an Inductive Signal for Salivary Amylase in Mesenchymal Stem Cells. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 30759717) »
- Horsley H et al. A urine-dependent human urothelial organoid offers a potential alternative to rodent models of infection. Sci Rep 8:1238 (2018). ICC/IF ; Human . Read more (PubMed: 29352171) »