Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (ab167566)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody
See all Muscarinic Acetylcholine Receptor M3/CHRM3 primary antibodies -
DescriptionMouse polyclonal to Muscarinic Acetylcholine Receptor M3/CHRM3
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Host speciesMouse
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Chimpanzee, Gorilla
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Immunogen
Full length protein corresponding to Human Muscarinic Acetylcholine Receptor M3/CHRM3 aa 1-590.
Sequence:MTLHNNSTTSPLFPNISSSWIHSPSDAGLPPGTVTHFGSYNVSRAAGNFS SPDGTTDDPLGGHTVWQVVFIAFLTGILALVTIIGNILVIVSFKVNKQLK TVNNYFLLSLACADLIIGVISMNLFTTYIIMNRWALGNLACDLWLAIDYV ASNASVMNLLVISFDRYFSITRPLTYRAKRTTKRAGVMIGLAWVISFVLW APAILFWQYFVGKRTVPPGECFIQFLSEPTITFGTAIAAFYMPVTIMTIL YWRIYKETEKRTKELAGLQASGTEAETENFVHPTGSSRSCSSYELQQQSM KRSNRRKYGRCHFWFTTKSWKPSSEQMDQDHSSSDSWNNNDAAASLENSA SSDEEDIGSETRAIYSIVLKLPGHSTILNSTKLPSSDNLQVPEEELGMVD LERKADKLQAQKSVDDGGSFPKSFSKLPIQLESAVDTAKTSDVNSSVGKS TATLPLSFKEATLAKRFALKTRSQITKRKRMSLVKEKKAAQTLSAILLAF IITWTPYNIMVLVNTFCDSCIPKTFWNLGYWLCYINSTVNPVCYALCNKT FRTTFKMLLLCQCDKKKRRKQQYQQRQSVIFHKRAPEQAL
Database link: NP_000731.1 -
Positive control
- HeLa cell lysate; Muscarinic Acetylcholine Receptor M3/CHRM3 transfected 293T cell lysate.
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General notes
This product was previously labelled as Muscarinic Acetylcholine Receptor M3
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferpH: 7.20
Constituent: 99% PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab167566 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 66 kDa. |
Target
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FunctionThe muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
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Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily.
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Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane. Basolateral cell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 469725 Chimpanzee
- Entrez Gene: 281685 Cow
- Entrez Gene: 101154508 Gorilla
- Entrez Gene: 1131 Human
- Entrez Gene: 12671 Mouse
- Entrez Gene: 100144478 Pig
- Entrez Gene: 24260 Rat
- Omim: 118494 Human
see all -
Alternative names
- AChR antibody
- ACM3_HUMAN antibody
- cholinergic receptor muscarinic 3 antibody
see all
Images
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Western blot - Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (ab167566)Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (ab167566) at 1 µg/ml + HeLa cell lysate at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 66 kDa -
Western blot - Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (ab167566)All lanes : Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (ab167566) at 1 µg/ml
Lane 1 : Muscarinic Acetylcholine Receptor M3/CHRM3 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 66 kDa
Protocols
Datasheets and documents
References
ab167566 has not yet been referenced specifically in any publications.
