Overview

  • Product name

    Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody [18C7.2]
    See all Muscarinic Acetylcholine Receptor M4/CHRM4 primary antibodies
  • Description

    Mouse monoclonal [18C7.2] to Muscarinic Acetylcholine Receptor M4/CHRM4
  • Host species

    Mouse
  • Specificity

    ab77956 reacts with Muscarinic Acetylcholine Receptor M4/CHRM4. There is no reactivity with the other subtypes.

  • Tested applications

    Suitable for: IHC-Fr, Flow Cyt, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Monkey
  • Immunogen

    Recombinant fragment corresponding to Human Muscarinic Acetylcholine Receptor M4/CHRM4.

  • Positive control

    • Transfected CHO cell lines
  • General notes

     This product was previously labelled as Muscarinic Acetylcholine Receptor M4

     This product was changed from ascites to tissue culture supernatant on 21 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab77956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 53 kDa.
IP Use at an assay dependent concentration.

Target

Images

  • Overlay histogram showing SH-SY5Y cells stained with ab77956 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77956, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This image was generated using the ascites version of the product.

References

This product has been referenced in:

  • Thomson SR  et al. Cell-Type-Specific Translation Profiling Reveals a Novel Strategy for Treating Fragile X Syndrome. Neuron 95:550-563.e5 (2017). Read more (PubMed: 28772121) »
  • Mei F  et al. Accelerated remyelination during inflammatory demyelination prevents axonal loss and improves functional recovery. Elife 5:N/A (2016). IHC-FoFr ; Mouse . Read more (PubMed: 27671734) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293, HGT101)
Loading amount
10 µg
Specification
HEK293, HGT101
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Dr. Rob Tolhurst

Verified customer

Submitted Sep 16 2010

Application
Western blot
Sample
Mouse Cell lysate - whole cell (STC1)
Loading amount
10 µg
Specification
STC1
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Dr. Rob Tolhurst

Verified customer

Submitted Sep 16 2010

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