Overview

  • Product name
    Anti-Muscarinic Acetylcholine Receptor M5 antibody
    See all Muscarinic Acetylcholine Receptor M5 primary antibodies
  • Description
    Rabbit polyclonal to Muscarinic Acetylcholine Receptor M5 - Carboxyterminal end
  • Host species
    Rabbit
  • Specificity
    Cross reactivity: M5 Muscarinic Receptor (519-531): 100% M5 Muscarinic Receptor: 75% No cross reactivity was observed in: M1 Muscarinic Receptor(451-460) M1 Muscarinic Receptor M2 Muscarinic Receptor(457-466) M2 Muscarinic Receptor M3 Muscarinic Receptor(580-589) M3 Muscarinic Receptor M4 Muscarinic Receptor(469-478) M4 Muscarinic Receptor
  • Tested applications
    Suitable for: WB, ELISA, IHC (PFA fixed), IHC-FoFrmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Synthetic peptide analogue of the C terminal of rat muscarinic M5 receptor covalently attached onto a carrier protein.

  • Positive control
    • Rat brain tissue, whole brain homogenate and cultured cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab41171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/400. Predicted molecular weight: 60 kDa. Blocking with 5% goat or donkey serum significantly reduces background when compared with BSA or milk blocking.
ELISA Use at an assay dependent concentration.
IHC (PFA fixed) 1/400.
IHC-FoFr Use at an assay dependent concentration. PubMed: 23773170

Target

  • Relevance
    The muscarinic acetylcholine receptor M5 is a member of the GPCR family (subfamily, acetylcholine) which mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. The rimary transducing effect is PI turnover. The receptor has been reported to be expressed in blood, brain, and ovary. ESTs have been isolated from human testis and placenta libraries.
  • Cellular localization
    Membrane; multi-pass membrane protein.
  • Database links
  • Alternative names
    • Acetylcholine receptor muscarinic 5 antibody
    • AChR antibody
    • AChR M5 antibody
    • CHKM5MR antibody
    • Cholinergic receptor muscarinic 5 antibody
    • Chrm 5 antibody
    • CHRM5 antibody
    • HM 5 antibody
    • HM5 antibody
    • m5 antibody
    • M5R antibody
    • MGC41838 antibody
    see all

References

This product has been referenced in:
  • Zhu H  et al. Impairments of spatial memory in an Alzheimer's disease model via degeneration of hippocampal cholinergic synapses. Nat Commun 8:1676 (2017). Read more (PubMed: 29162816) »
  • Muschter D  et al. Reactivity of rat bone marrow-derived macrophages to neurotransmitter stimulation in the context of collagen II-induced arthritis. Arthritis Res Ther 17:169 (2015). IF . Read more (PubMed: 26104678) »
See all 4 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Vielen Dank für Ihre Anfrage.

Leider bieten wir generell keine kostenlosen Proben zu Testzwecken an. Hier bei Abcam verfahren wir so, dass wir einen kostenlosen Ersatz oder eine Gutschrift verschicken, falls ein Antikörper nicht so funktioniert wie auf unserem Datenblatt beschrieben. Falls der Antikörper in einer bisher nicht getesteten Anwendung oder Spezies verwendet wird, können wir leider keinen Ersatz anbieten, wir möchten Sie jedoch einladen unser Abreview-System zu verwenden.

https://www.abcam.com/abtrial

Das Abreview-System erlaubt es unseren Kunden, Rezensionen unserer Produkte zu veröffendlichen und dafür Punkte zu erhalten. Diese “Abpoints” können gegen Rabatte beim Kauf weiterer Produkte oder Geschenkgutscheine wie z.B. Amazon.com-Gutscheine eingetauscht werden. Sollten Sie sich dazu entscheiden, dieses Produkt zu kaufen, teilen Sie uns bitte Ihre Ergebnisse mittels eines „Abreview“ mit. Sie können die Rezension durch einen Link direkt auf unserem Produktdatenblatt abschicken.


Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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Answer

Thank you for your previous email, and I sincerely apologize for the extended delay getting back to you.

I have not been able to obtain any images of this antibody using in tissue staining, and I am sorry that we don't have these available.

As I mentioned before, the antibody has only been tested in Western blot, ELISA, and IHC on standard PFA-fixed tissue.We do fully guarantee the antibody to work in these tested applications, however if you would like to try the antibody in IHC of PFA-perfused tissue (IHC-FoFr), we do have a testing discount program that you might be interested in. In this program, you would purchase ab41171 and test it in IHC-FoFr, then after submitting an Abreview with your data and protocol, we will give you one free primary antibody on a future order. I'd be happy to send more information if you are interested in the program.

Please let me know if you have any additional questions or if there is anything else that we can do for you.

Read More

Answer

Thank you for contacting us with your question.

I've looked through our records andto our knowledge, the antibody has only been tested inWestern blot, ELISA, and IHC on standard PFA-fixed tissue. I will get in touch with the lab and request images from the antibody testing in these applications.

We do fully guarantee the antibody to work in these tested applications, however if you would like to try the antibody in IHC of PFA-perfused tissue (IHC-FoFr), we do have a testing discount program that you might be interested in. In this program, you would purchase ab41171 and test it in IHC-FoFr, then after submitting an Abreview with your data and protocol, we will give you one free primary antibody on a future order. I'd be happy to send more information if you are interested in the program.

I'll be back in touch once I hear from the lab. Please let me know if you have any questions or if there is anything else we can do for you in the meantime.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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