Anti-Muscarinic Acetylcholine Receptor M5/CHRM5 antibody (ab186830)
Key features and details
- Rabbit polyclonal to Muscarinic Acetylcholine Receptor M5/CHRM5
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Muscarinic Acetylcholine Receptor M5/CHRM5 antibody
See all Muscarinic Acetylcholine Receptor M5/CHRM5 primary antibodies -
Description
Rabbit polyclonal to Muscarinic Acetylcholine Receptor M5/CHRM5 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human Muscarinic Acetylcholine Receptor M5/CHRM5 aa 230-430 (internal sequence).
Database link: P08912 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186830 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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WB |
1/500 - 1/2000. Predicted molecular weight: 60 kDa.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
WB
1/500 - 1/2000. Predicted molecular weight: 60 kDa. |
Target
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Function
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM5 sub-subfamily. -
Cellular localization
Cell membrane. Cell junction, synapse, postsynaptic cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1133 Human
- Entrez Gene: 213788 Mouse
- Omim: 118496 Human
- SwissProt: P08912 Human
- SwissProt: A2AHU3 Mouse
- SwissProt: Q920H4 Mouse
- Unigene: 584747 Human
- Unigene: 297624 Mouse
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Alternative names
- Acetylcholine receptor muscarinic 5 antibody
- AChR antibody
- AChR M5 antibody
see all
Images
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Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186830. Blue DAPI for nuclear staining.
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All lanes : Anti-Muscarinic Acetylcholine Receptor M5/CHRM5 antibody (ab186830) at 1/500 dilution
Lane 1 : BT474 cell extract
Lane 2 : U251 cell extract
Lane 3 : Mouse liver extract
Predicted band size: 60 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab186830 has not yet been referenced specifically in any publications.