RecombinantRabMAb

Recombinant Anti-muscle Actin antibody [EP184E] - BSA and Azide free (ab247305)

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Western blot - Anti-muscle Actin antibody [EP184E] - BSA and Azide free (ab247305)
  • Immunocytochemistry - Anti-muscle Actin antibody [EP184E] - BSA and Azide free (ab247305)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EP184E] - BSA and Azide free (ab247305)
  • Flow Cytometry - Anti-muscle Actin antibody [EP184E] - BSA and Azide free (ab247305)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EP184E] - BSA and Azide free (ab247305)
  • Anti-muscle Actin antibody [EP184E] - BSA and Azide free (ab247305)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP184E] to muscle Actin - BSA and Azide free
  • Suitable for: IHC-P, IHC-FoFr, Flow Cyt, WB, ICC
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-muscle Actin antibody [EP184E] - BSA and Azide free
    See all muscle Actin primary antibodies

  • Description

    Rabbit monoclonal [EP184E] to muscle Actin - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This Actin antibody recognizes all muscle actin.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247305 is the carrier-free version of ab46805. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab247305 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab247305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
IHC-P
Human
WB
Human
All applications
Mouse
Cow
Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-FoFr
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
ICC
Use at an assay dependent concentration.
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-FoFr
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
ICC
Use at an assay dependent concentration.

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

  • Involvement in disease

    Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy, actin, congenital, with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.

  • Sequence similarities

    Belongs to the actin family.

  • Post-translational
    modifications

    Oxidation of Met-46 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced.

  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • a actin antibody
    • ACTA antibody
    • ACTA1 antibody
    • ACTA2 antibody
    • ACTC antibody
    • ACTC1 antibody
    • Actin antibody
    • ACTS_HUMAN antibody
    • ACTSA antibody
    • Alpha 2 actin antibody
    • alpha skeletal muscle antibody
    • Alpha-actin-1 antibody
    • Cardiac muscle alpha actin 1 antibody
    • Skeletal muscle alpha actin 1 antibody
    see all

Images

  • Western blot - Anti-muscle Actin antibody [EP184E] - BSA and Azide free (ab247305)
    Western blot - Anti-muscle Actin antibody [EP184E] - BSA and Azide free (ab247305)
    Anti-muscle Actin antibody [EP184E] (ab46805) at 1/1000 dilution + A431 cell lysate at 10ug/lane

    Secondary
    Goat anti-rabbit, HRP labelled. at 1/2000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa



    This data was developed using ab46805, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-muscle Actin antibody [EP184E] - BSA and Azide free (ab247305)
    Immunocytochemistry - Anti-muscle Actin antibody [EP184E] - BSA and Azide free (ab247305)
    This data was developed using ab46805, the same antibody clone in a different buffer formulation.Immunofluorescent staining of HeLa cells using ab46805 at a 1/250 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EP184E] - BSA and Azide free (ab247305)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EP184E] - BSA and Azide free (ab247305)
    This data was developed using ab46805, the same antibody clone in a different buffer formulation.Immunohistochemical staining of paraffin-embedded human cardiac muscle using anti-Actin ab46805 at a 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-muscle Actin antibody [EP184E] - BSA and Azide free (ab247305)
    Flow Cytometry - Anti-muscle Actin antibody [EP184E] - BSA and Azide free (ab247305)
    This data was developed using ab46805, the same antibody clone in a different buffer formulation.Overlay histogram showing HeLa cells stained with ab46805 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab46805, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EP184E] - BSA and Azide free (ab247305)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EP184E] - BSA and Azide free (ab247305)
    This data was developed using ab46805, the same antibody clone in a different buffer formulation.Fluorescent immunohistochemical analysis of paraffin-embedded human smooth muscle tissue using ab46805. Green-specific fluorescent staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-muscle Actin antibody [EP184E] - BSA and Azide free (ab247305)
    Anti-muscle Actin antibody [EP184E] - BSA and Azide free (ab247305)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab247305? Please let us know so that we can cite the reference in this datasheet.

ab247305 has not yet been referenced specifically in any publications.

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