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Tags & Cell Markers Subcellular Markers Cytoskeleton Actin
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RecombinantRabMAb

Recombinant Anti-muscle Actin antibody [EPR8484] (ab156302)

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Western blot - Anti-muscle Actin antibody [EPR8484] (ab156302)
  • Flow Cytometry - Anti-muscle Actin antibody [EPR8484] (ab156302)
  • Western blot - Anti-muscle Actin antibody [EPR8484] (ab156302)
  • Western blot - Anti-muscle Actin antibody [EPR8484] (ab156302)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EPR8484] (ab156302)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EPR8484] (ab156302)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EPR8484] (ab156302)
  • Immunocytochemistry/ Immunofluorescence - Anti-muscle Actin antibody [EPR8484] (ab156302)
  • Flow Cytometry - Anti-muscle Actin antibody [EPR8484] (ab156302)
  • Immunoprecipitation - Anti-muscle Actin antibody [EPR8484] (ab156302)
  • Western blot - Anti-muscle Actin antibody [EPR8484] (ab156302)
  • Western blot - Anti-muscle Actin antibody [EPR8484] (ab156302)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EPR8484] (ab156302)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EPR8484] (ab156302)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EPR8484] (ab156302)
  • Immunocytochemistry/ Immunofluorescence - Anti-muscle Actin antibody [EPR8484] (ab156302)
  • Immunocytochemistry/ Immunofluorescence - Anti-muscle Actin antibody [EPR8484] (ab156302)
  • Flow Cytometry - Anti-muscle Actin antibody [EPR8484] (ab156302)
  • OI-RD Scanning - Anti-muscle Actin antibody [EPR8484] (ab156302)
  • Anti-muscle Actin antibody [EPR8484] (ab156302)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8484] to muscle Actin
  • Suitable for: IHC-P, WB, ICC/IF, IP, Flow Cyt
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-muscle Actin antibody [EPR8484]
    See all muscle Actin primary antibodies
  • Description

    Rabbit monoclonal [EPR8484] to muscle Actin
  • Host species

    Rabbit
  • Specificity

    ab156302 will detect alpha and gamma specific actin from skeletal, cardiac and smooth muscle.
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Mouse
    Human
    IHC-P
    Mouse
    Rat
    Human
    IP
    Mouse
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human muscle Actin aa 350 to the C-terminus. The exact sequence is proprietary.
    Database link: P63267

  • Positive control

    • WB: HeLa, A431, A673, HEK293, MCF7, L6 and NIH 3T3 cell lysates, human fetal artery, kidney and heart tissue lysates, human uterus, stomach and skeletal muscle tissue lysates and rat spleen tissue lysates. IHC-P: Human skeletal muscle, smooth muscle in colon, cervical carcinoma and heart muscle tissues. Mouse cardiac muscle and rat colon tissues. ICC/IF: HeLa, A673 and NIH3T3 cells. Flow Cyt: HeLa cells. IP: NIH 3T3 cell lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8484
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Actin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Troponin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Tropomyosin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Crosslinking
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Assembly
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Death receptors & ligands
    • RIP
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Death receptors & ligands
    • RIP

Associated products

  • Alternative Versions

    • HRP Anti-muscle Actin antibody [EPR8484] - Loading Control (ab185058)
    • Alexa Fluor® 488 Anti-muscle Actin antibody [EPR8484] (ab190196)
    • Alexa Fluor® 647 Anti-muscle Actin antibody [EPR8484] (ab190567)
    • Anti-muscle Actin antibody [EPR8484] - BSA and Azide free (ab224207)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • MCF7 whole cell lysate (ab29537)
    • HeLa whole cell lysate (ab29545)
    • A-431 whole cell lysate (ab30132)
    • NIH/3T3 whole cell lysate (ab7179)
    • HEK-293 whole cell lysate (ab7902)
    • A-431 whole cell lysate (ab7909)
  • Related Products

    • Wiskostatin, N-WASP inhibitor (ab141085)
    • CK 666, Actin polymerization inhibitor (ab141231)
    • Jasplakinolide, Actin polymerization and stabilization inducer (ab141409)
    • Cytochalasin E, actin-polymerization inhibitor (ab141788)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab156302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Mouse
Human
IHC-P
Mouse
Rat
Human
IP
Mouse
WB
Mouse
Human
Application Abreviews Notes
IHC-P
1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB (1)
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
ICC/IF
1/100 - 1/250.
IP
1/10 - 1/100.
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Notes
IHC-P
1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
ICC/IF
1/100 - 1/250.
IP
1/10 - 1/100.
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy, actin, congenital, with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
  • Sequence similarities

    Belongs to the actin family.
  • Post-translational
    modifications

    Oxidation of Met-46 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P68133 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 58 Human
    • Entrez Gene: 11459 Mouse
    • Entrez Gene: 29437 Rat
    • Omim: 102610 Human
    • SwissProt: P68133 Human
    • SwissProt: P68033 Mouse
    • SwissProt: P68134 Mouse
    • SwissProt: P68136 Rat
    • Unigene: 1288 Human
    • Unigene: 214950 Mouse
    • Unigene: 82732 Rat
    see all
  • Alternative names

    • a actin antibody
    • ACTA antibody
    • ACTA1 antibody
    • ACTA2 antibody
    • ACTC antibody
    • ACTC1 antibody
    • Actin antibody
    • ACTS_HUMAN antibody
    • ACTSA antibody
    • Alpha 2 actin antibody
    • alpha skeletal muscle antibody
    • Alpha-actin-1 antibody
    • Cardiac muscle alpha actin 1 antibody
    • Skeletal muscle alpha actin 1 antibody
    see all

Images

  • Western blot - Anti-muscle Actin antibody [EPR8484] (ab156302)
    Western blot - Anti-muscle Actin antibody [EPR8484] (ab156302)
    All lanes : Anti-muscle Actin antibody [EPR8484] (ab156302) at 1/10000 dilution (purified)

    Lane 1 : NIH/3T3 whole cell lysate
    Lane 2 : Rat spleen tissue lysate
    Lane 3 : L6 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/10000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa



    Blocking and dilution buffer: 5% NFDM /TBST.

  • Flow Cytometry - Anti-muscle Actin antibody [EPR8484] (ab156302)
    Flow Cytometry - Anti-muscle Actin antibody [EPR8484] (ab156302)
    Overlay histogram showing HeLa cells fixed in 4% PFA and stained with purified ab156302 at a dilution of 1 in 50 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).
  • Western blot - Anti-muscle Actin antibody [EPR8484] (ab156302)
    Western blot - Anti-muscle Actin antibody [EPR8484] (ab156302)
    Anti-muscle Actin antibody [EPR8484] (ab156302) at 1/10000 dilution (purified) + Human stomach tissue lysate at 10 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/10000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa



    Blocking and dilution buffer: 5% NFDM /TBST.

  • Western blot - Anti-muscle Actin antibody [EPR8484] (ab156302)
    Western blot - Anti-muscle Actin antibody [EPR8484] (ab156302)
    Anti-muscle Actin antibody [EPR8484] (ab156302) at 1/1000 dilution (purified) + A673 whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa



    Blocking and dilution buffer: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EPR8484] (ab156302)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EPR8484] (ab156302)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue labelling muscle Actin with purified ab156302 at a dilution of 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EPR8484] (ab156302)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EPR8484] (ab156302)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue labelling muscle Actin with purified ab156302 at a dilution of 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EPR8484] (ab156302)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EPR8484] (ab156302)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labelling muscle Actin with purified ab156302 at a dilution of 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-muscle Actin antibody [EPR8484] (ab156302)
    Immunocytochemistry/ Immunofluorescence - Anti-muscle Actin antibody [EPR8484] (ab156302)

    Immunocytochemistry/Immunofluorescence analysis of A673 cells labelling muscle Actin with purified ab156302 at 1/100. Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

  • Flow Cytometry - Anti-muscle Actin antibody [EPR8484] (ab156302)
    Flow Cytometry - Anti-muscle Actin antibody [EPR8484] (ab156302)

    Flow Cytometry analysis of HeLa cells labelling muscle Actin with purified ab156302 at 1/50 (red). Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • Immunoprecipitation - Anti-muscle Actin antibody [EPR8484] (ab156302)
    Immunoprecipitation - Anti-muscle Actin antibody [EPR8484] (ab156302)

    ab156302 (purified) at 1/20 immunoprecipitating muscle Actin in NIH/3T3 whole cell lysate.

    Lane 1 (input): NIH/3T3 whole cell lysate (10µg)

    Lane 2 (+): ab156302 + NIH/3T3 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab156302 in NIH/3T3 whole cell lysate.

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-muscle Actin antibody [EPR8484] (ab156302)
    Western blot - Anti-muscle Actin antibody [EPR8484] (ab156302)
    All lanes : Anti-muscle Actin antibody [EPR8484] (ab156302) at 1/1000 dilution (unpurified)

    Lane 1 : NIH 3T3 lysate
    Lane 2 : Human fetal artery lysate
    Lane 3 : Human fetal kidney lysate
    Lane 4 : Human uterus lysate
    Lane 5 : Human stomach lysate
    Lane 6 : Human fetal heart lysate
    Lane 7 : Human skeletal muscle lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 42 kDa

  • Western blot - Anti-muscle Actin antibody [EPR8484] (ab156302)
    Western blot - Anti-muscle Actin antibody [EPR8484] (ab156302)
    All lanes : Anti-muscle Actin antibody [EPR8484] (ab156302) at 1/1000 dilution (unpurified)

    Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue
    Lane 6 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa



    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab156302 overnight at 4°C. Antibody binding was detected using ab175781 at a 1/10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

    Secondary antibody - goat anti-rabbit Alexa Fluor® 790 (ab175781).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EPR8484] (ab156302)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EPR8484] (ab156302)

    IHC image of unpurified ab156302 staining muscle Actin in human colon* formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with unpurified ab156302, 5μg/ml working concentration, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the secondary only control (shown on the inset).
    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EPR8484] (ab156302)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EPR8484] (ab156302)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling muscle Actin with unpurified ab156302 at a dilution of 1/1000.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EPR8484] (ab156302)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EPR8484] (ab156302)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart muscle tissue labelling muscle Actin with unpurified ab156302 at a dilution of 1/1000.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-muscle Actin antibody [EPR8484] (ab156302)
    Immunocytochemistry/ Immunofluorescence - Anti-muscle Actin antibody [EPR8484] (ab156302)

    Unpurified ab156302 staining Actin in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with unpurified ab156302 at 10μg/ml and ab7291 at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an goat anti-rabbit Alexa Fluor® 488 secondary (ab150081) at 2 μg/ml (shown in green) and goat anti-mouse Alexa Fluor® 594 secondary (ab150120) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.

    Negative controls: 1 – Rabbit primary and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

  • Immunocytochemistry/ Immunofluorescence - Anti-muscle Actin antibody [EPR8484] (ab156302)
    Immunocytochemistry/ Immunofluorescence - Anti-muscle Actin antibody [EPR8484] (ab156302)

    Unpurified ab156302 staining Actin in NIH3T3 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with unpurified ab156302 at 5μg/ml and ab195889 at 1/250 dilution (shown in pseudo color red) overnight at +4°C, followed by a further incubation at room temperature for 1h with an goat anti-rabbit Alexa Fluor® 488 secondary (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Flow Cytometry - Anti-muscle Actin antibody [EPR8484] (ab156302)
    Flow Cytometry - Anti-muscle Actin antibody [EPR8484] (ab156302)

    Overlay histogram showing HeLa cells stained with unpurified ab156302 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (unpurified ab156302, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was goat anti-rabbit Alexa Fluor® 488 (IgG H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • OI-RD Scanning - Anti-muscle Actin antibody [EPR8484] (ab156302)
    OI-RD Scanning - Anti-muscle Actin antibody [EPR8484] (ab156302)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-muscle Actin antibody [EPR8484] (ab156302)
    Anti-muscle Actin antibody [EPR8484] (ab156302)

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (8)

    Publishing research using ab156302? Please let us know so that we can cite the reference in this datasheet.

    ab156302 has been referenced in 8 publications.

    • Li Y  et al. Transcription factor TBX18 promotes adult rat bone mesenchymal stem cell differentiation to biological pacemaker cells. Int J Mol Med 41:845-851 (2018). PubMed: 29207072
    • Solomon O  et al. RNA editing by ADAR1 leads to context-dependent transcriptome-wide changes in RNA secondary structure. Nat Commun 8:1440 (2017). PubMed: 29129909
    • Fischer U  et al. Gene amplification during myogenic differentiation. Oncotarget 7:6864-77 (2016). PubMed: 26760505
    • Zhao Y  et al. Environmental Enrichment Attenuated Sevoflurane-Induced Neurotoxicity through the PPAR-? Signaling Pathway. Biomed Res Int 2015:107149 (2015). WB ; Mouse . PubMed: 26236713
    • Song Q  et al. Sevoflurane induces neurotoxicity in young mice through FAS/FASL signaling. Genet Mol Res 14:18059-68 (2015). PubMed: 26782453
    • Han B  et al. Protective effects of tetrandrine on brain cells in phenobarbital-dependent and -withdrawn rats. Mol Med Rep 11:1939-44 (2015). PubMed: 25434925
    • Chen S  et al. Identification of HnRNP M as a novel biomarker for colorectal carcinoma by quantitative proteomics. Am J Physiol Gastrointest Liver Physiol 306:G394-403 (2014). PubMed: 24381081
    • Yang JM  et al. Angiotensin-(1-7) dose-dependently inhibits atherosclerotic lesion formation and enhances plaque stability by targeting vascular cells. Arterioscler Thromb Vasc Biol 33:1978-85 (2013). IHC (PFA fixed) ; Mouse . PubMed: 23723368

    Customer reviews and Q&As

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    Western blot abreview for Anti-Actin antibody [EPR8484]

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    15 µg
    Gel Running Conditions
    Reduced Denaturing (13%)
    Sample
    Human Cell lysate - whole cell (HCT116 and MEF cell lysate)
    Specification
    HCT116 and MEF cell lysate
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Mr. Christian Marx

    Verified customer

    Submitted Feb 21 2014

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