• Product name

    Anti-muscle Actin antibody [HHF35]
    See all muscle Actin primary antibodies
  • Description

    Mouse monoclonal [HHF35] to muscle Actin
  • Host species

  • Specificity

    This antibody recognizes muscle specific alpha and gamma actin isomers but is non-reactive with beta isomers.
  • Tested applications

    Suitable for: WB, IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    The SDS extracted protein fraction of human myocardium.

  • General notes

    This antibody stains myocardial, skeletal muscle and smooth muscle cells as well as myoepithelial cells, pericytes of small vessels. All other non-muscle cells were negative.


  • Form

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.1% Sodium azide
  • Purity

    Tissue culture supernatant
  • Primary antibody notes

    This antibody stains myocardial, skeletal muscle and smooth muscle cells as well as myoepithelial cells, pericytes of small vessels. All other non-muscle cells were negative.
  • Clonality

  • Clone number

  • Isotype

  • Light chain type

  • Research areas


Our Abpromise guarantee covers the use of ab7813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 42 kDa. PubMed: 24284066
IHC-P 1/25 - 1/75.

Prolonged fixation in buffered formalin can destroy the epitope.

IHC-Fr Use at an assay dependent concentration.


  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy, actin, congenital, with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
  • Sequence similarities

    Belongs to the actin family.
  • Post-translational

    Oxidation of Met-46 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • a actin antibody
    • ACTA antibody
    • ACTA1 antibody
    • ACTA2 antibody
    • ACTC antibody
    • ACTC1 antibody
    • Actin antibody
    • ACTS_HUMAN antibody
    • ACTSA antibody
    • Alpha 2 actin antibody
    • alpha skeletal muscle antibody
    • Alpha-actin-1 antibody
    • Cardiac muscle alpha actin 1 antibody
    • Skeletal muscle alpha actin 1 antibody
    see all


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle labelling muscle Actin with ab7813.

  • Ab7813 staining human normal skeletal muscle. Staining is localized to the cytoplasm.
    Left panel: with primary antibody diluted 1:4000. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.


This product has been referenced in:

  • Xia D  et al. miR302a inhibits the proliferation of esophageal cancer cells through the MAPK and PI3K/Akt signaling pathways. Oncol Lett 15:3937-3943 (2018). Read more (PubMed: 29456742) »
  • Hoffmann C  et al. Live cell imaging reveals actin-cytoskeleton-induced self-association of the actin-bundling protein WLIM1. J Cell Sci 127:583-98 (2014). WB ; Human . Read more (PubMed: 24284066) »
See all 7 Publications for this product

Customer reviews and Q&As


It has not been tested on mouse so we do not know how it will react, however as it was raised in mouse we believe the background would be too strong.

Read More

For licensing inquiries, please contact partnerships@abcam.com

Sign up