Key features and details
- Mouse monoclonal [MSA/953] to muscle Actin
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG1
Product nameAnti-muscle Actin antibody [MSA/953]
See all muscle Actin primary antibodies
DescriptionMouse monoclonal [MSA/953] to muscle Actin
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Rabbit
Synthetic peptide within Human muscle Actin. The exact sequence is proprietary. (Common among human muscle Actins. UniProt P62736, P68032 and P68133).
- Human rhabdomyosarcoma and leiomyosarcoma tissues; Rat lung, heart, stomach and skeletal muscle tissues; hSKM cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Concentration information loading...
PurityProtein G purified
Purification notesab216039 was purified from Bioreactor Concentrate by Protein A/G.
Light chain typekappa
Our Abpromise guarantee covers the use of ab216039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 0.25 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
(Primary incubation for 30 minutes at RT).
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 42 kDa.|
FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Involvement in diseaseDefects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
Defects in ACTA1 are a cause of myopathy, actin, congenital, with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
Sequence similaritiesBelongs to the actin family.
modificationsOxidation of Met-46 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- a actin antibody
- ACTA antibody
- ACTA1 antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human rhabdomyosarcoma tissue labeling muscle Actin with ab216039 at 0.5 µg/ml.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human leiomyosarcoma tissue labeling muscle Actin with ab216039 at 0.5 µg/ml.
Anti-muscle Actin antibody [MSA/953] (ab216039) at 1 µg/ml + hSKM cell lysate
Predicted band size: 42 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat lung tissue labeling muscle Actin with ab216039 at 0.5 µg/ml.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat heart tissue labeling muscle Actin with ab216039 at 0.5 µg/ml.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat stomach tissue labeling muscle Actin with ab216039 at 0.5 µg/ml.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat skeletal muscle tissue labeling muscle Actin with ab216039 at 0.5 µg/ml.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab216039 has not yet been referenced specifically in any publications.