Overview

  • Product name

    Anti-muscle Actin antibody [MSA/953]
    See all muscle Actin primary antibodies
  • Description

    Mouse monoclonal [MSA/953] to muscle Actin
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Rabbit
  • Immunogen

    Synthetic peptide within Human muscle Actin. The exact sequence is proprietary. (Common among human muscle Actins. UniProt P62736, P68032 and P68133).

  • Positive control

    • Human rhabdomyosarcoma and leiomyosarcoma tissues; Rat lung, heart, stomach and skeletal muscle tissues; hSKM cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    ab216039 was purified from Bioreactor Concentrate by Protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    MSA/953
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab216039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.25 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

(Primary incubation for 30 minutes at RT).

WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 42 kDa.

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy, actin, congenital, with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
  • Sequence similarities

    Belongs to the actin family.
  • Post-translational
    modifications

    Oxidation of Met-46 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • a actin antibody
    • ACTA antibody
    • ACTA1 antibody
    • ACTA2 antibody
    • ACTC antibody
    • ACTC1 antibody
    • Actin antibody
    • ACTS_HUMAN antibody
    • ACTSA antibody
    • Alpha 2 actin antibody
    • alpha skeletal muscle antibody
    • Alpha-actin-1 antibody
    • Cardiac muscle alpha actin 1 antibody
    • Skeletal muscle alpha actin 1 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human rhabdomyosarcoma tissue labeling muscle Actin with ab216039 at 0.5 µg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human leiomyosarcoma tissue labeling muscle Actin with ab216039 at 0.5 µg/ml.

  • Anti-muscle Actin antibody [MSA/953] (ab216039) at 1 µg/ml + hSKM cell lysate

    Predicted band size: 42 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat lung tissue labeling muscle Actin with ab216039 at 0.5 µg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat heart tissue labeling muscle Actin with ab216039 at 0.5 µg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat stomach tissue labeling muscle Actin with ab216039 at 0.5 µg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat skeletal muscle tissue labeling muscle Actin with ab216039 at 0.5 µg/ml.

References

ab216039 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab216039.
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