• Product name
  • Description
    Rabbit polyclonal to MUSK
  • Host species
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Does not react with: Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human MUSK.

  • Positive control
    • This antibody gave a positive signal in Human and Mouse Skeletal Muscle Tissue Lysates.



Our Abpromise guarantee covers the use of ab92950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 97 kDa).


  • Function
    Receptor tyrosine kinase that is a key mediator of agrin's action and is involved in neuromuscular junction (NMJ) organization.
  • Involvement in disease
    Defects in MUSK is a cause of congenital myasthenic syndrome with acetylcholine receptor deficiency (CMS-ACHRD) [MIM:608931]. A post-synaptic congenital myasthenic syndrome. Mutations underlying AChR deficiency cause a 'loss of function' and show recessive inheritance. Note=MUSK mutations lead to decreased agrin-dependent AChR aggregation, a critical step in the formation of the neuromuscular junction.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family.
    Contains 1 FZ (frizzled) domain.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    Ubiquitinated by PDZRN3. Ubiquitination promotes endocytosis and lysosomal degradation.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • CMS9 antibody
    • FADS antibody
    • MDK 4 antibody
    • MDK4 antibody
    • MGC126323 antibody
    • MGC126324 antibody
    • Muscle antibody
    • Muscle associated receptor tyrosine kinase antibody
    • Muscle skeletal receptor tyrosine kinase antibody
    • Muscle skeletal receptor tyrosine protein kinase antibody
    • Muscle specific kinase receptor antibody
    • Muscle specific tyrosine kinase receptor antibody
    • Muscle specific tyrosine protein kinase receptor antibody
    • Muscle-specific kinase receptor antibody
    • Muscle-specific tyrosine-protein kinase receptor antibody
    • MuSK antibody
    • MUSK_HUMAN antibody
    • Neural fold somite kinase 1 antibody
    • Neural fold somite kinase 2 antibody
    • Neural fold somite kinase 3 antibody
    • Neural fold somite kinase1 antibody
    • Neural fold somite kinase2 antibody
    • Neural fold somite kinase3 antibody
    • Nsk 1 antibody
    • Nsk 2 antibody
    • Nsk 3 antibody
    • Nsk1 antibody
    • Nsk2 antibody
    • Nsk3 antibody
    • Receptor tyrosine kinase MuSK antibody
    • Skeletal muscle receptor tyrosine kinase antibody
    • skeletal receptor tyrosine-protein kinase antibody
    see all


  • All lanes : Anti-MUSK antibody (ab92950) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 97 kDa
    Observed band size: 105 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 65 kDa, 80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 2 minutes

    MUSK protein sequence contains two potential glycosylation sites (SwissProt Data), which may explain the migration of the band at a higher molecular weight than predicted.


This product has been referenced in:
  • Logan CV  et al. Congenital Myasthenic Syndrome Type 19 Is Caused by Mutations in COL13A1, Encoding the Atypical Non-fibrillar Collagen Type XIII a1 Chain. Am J Hum Genet 97:878-85 (2015). Read more (PubMed: 26626625) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Western blot
Rat Cell lysate - whole cell (HEK293 cells transfected with rat MuSK)
Loading amount
30 µg
HEK293 cells transfected with rat MuSK
Gel Running Conditions
Reduced Denaturing (7,5% polyacrylamide)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 06 2012

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Date d’expiration : JJ MM AAAA

Je suis ravi que vous souhaitiez participer à notre offre et tester ab92950 chez le rat (80% d'homologie entre l'immunogène et la protéine de rat, voir fic...

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