Key features and details
- Rabbit polyclonal to MUSK - N-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MUSK antibody - N-terminal
See all MUSK primary antibodies
DescriptionRabbit polyclonal to MUSK - N-terminal
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Recombinant fragment within Human MUSK (N terminal). The exact sequence is proprietary.
Database link: O15146
- WB: NT2/D1 whole cell lysate. IHC-P: Human breast cancer tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228488 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 97 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionReceptor tyrosine kinase that is a key mediator of agrin's action and is involved in neuromuscular junction (NMJ) organization.
Involvement in diseaseDefects in MUSK is a cause of congenital myasthenic syndrome with acetylcholine receptor deficiency (CMS-ACHRD) [MIM:608931]. A post-synaptic congenital myasthenic syndrome. Mutations underlying AChR deficiency cause a 'loss of function' and show recessive inheritance. Note=MUSK mutations lead to decreased agrin-dependent AChR aggregation, a critical step in the formation of the neuromuscular junction.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family.
Contains 1 FZ (frizzled) domain.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
modificationsUbiquitinated by PDZRN3. Ubiquitination promotes endocytosis and lysosomal degradation.
- Information by UniProt
- CMS9 antibody
- FADS antibody
- MDK 4 antibody
Anti-MUSK antibody - N-terminal (ab228488) at 1/1000 dilution + NT2/D1 (human embryonal testis carcinoma cell line) whole cell lysate
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 97 kDa
7.5% SDS PAGE
Paraffin-embedded human breast cancer tissue stained for MUSK using ab228488 at 1/250 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228488 has not yet been referenced specifically in any publications.