Asynchronous cells were paraformaldehyde-fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. Under these conditions, this antibody failed to produce the expected staining pattern as extremely long exposure times were required (>1.5sec). MeOH-fixed samples were also evaluated and failed to produce the expected staining pattern.
Thank you for your valuable feedback. ICC/IF was not a tested application for ab32049 and it seems that this antibody does not recognize p53 under these experimental conditions. Thank you for your time - we hope that this information will be helpful for future customers.
Dr. Kirk Mcmanus
Submitted Jun 08 2012
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