Immunocytochemistry/ Immunofluorescence abreview for Anti-p53 antibody [Y5]

Poor
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde

Other product details

Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-Rabbit Alexafluor488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200

Additional data

Additional Notes
Asynchronous cells were paraformaldehyde-fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. Under these conditions, this antibody failed to produce the expected staining pattern as extremely long exposure times were required (>1.5sec). MeOH-fixed samples were also evaluated and failed to produce the expected staining pattern.
Abcam response
Thank you for your valuable feedback. ICC/IF was not a tested application for ab32049 and it seems that this antibody does not recognize p53 under these experimental conditions. Thank you for your time - we hope that this information will be helpful for future customers.

Dr. Kirk Mcmanus

Verified customer

Submitted Jun 08 2012

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