HCT116 p53wt treated with CPT-11 (7.5 ´M for 24 hrs) lysates
Specific signal at 55 kDa only after CPT-11 treatment, which induces p53 expression. Intense unspecific signal in both HCT116 p53wt and p53-/- cells at 35 and 40 kDa. Specificity of ab32049 is reduced compared to a non Abcam anti-p53 antibody. ab32049 additionally does not detect murine p53.
Thank you for your valuable feedback. ab32049 has been used to detect p53 in Human lung adenocarcinoma (A549) cells by Western blot. Your data suggests that ab32049 may not be suitable to detect endogenous p53 - without induction - by WB in Human cell lines under these experimental conditions. We hope this information is useful to our customers and we welcome further feedback.
Mr. Christian Marx
Submitted Jan 16 2014
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