Western blot abreview for Anti-p53 [Y5] antibody

Good
Abreviews
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (HepG2)
Specification
HepG2
Treatment
375uM 5-fluorouracil
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST + 2.5% milk
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
ECL
Bands
Specific: 53 kDa Non-specific: 45 kDa
Exposure
30 second(s)
Negative control
Ct: untreated cells
Positive control
Treated cells for up to 24 hours

Additional data

Additional Notes
A strong band was detected at 43 kDa

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Submitted Mar 27 2014

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