Product nameAnti-Muted antibody [EPR11081(B)]
DescriptionRabbit monoclonal [EPR11081(B)] to Muted
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Pig
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Muted aa 150 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q8TDH9
- U87-MG, 293T and HeLa cell lysate
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab175205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 22 kDa.|
|IP||1/10 - 1/100.|
FunctionThe BLOC-1 complex is required for normal biogenesis of lysosome-related organelles, such as platelet dense granules and melanosomes. Plays a role in intracellular vesicle trafficking.
Sequence similaritiesBelongs to the Muted family.
- Information by UniProt
- Biogenesis of lysosomal organelles complex 1, subunit 5, muted antibody
- Biogenesis of lysosome related organelles complex 1 subunit 5 antibody
- BLOC 1 subunit 5 antibody
All lanes : Anti-Muted antibody [EPR11081(B)] (ab175205) at 1/1000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 22 kDa
Western blot analysis on immunoprecipitation pellet from HeLa cell lysate (lane 1) labeling Muted with ab175205 at 1/10 dilution or 1X PBS (lane 2) negative control. HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.
ab175205 has not yet been referenced specifically in any publications.