Overview

  • Product name

    Anti-MVD antibody [EPR6559(2)]
    See all MVD primary antibodies
  • Description

    Rabbit monoclonal [EPR6559(2)] to MVD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MVD aa 250-350. The exact sequence is proprietary.

  • Positive control

    • Human fetal liver, K562 and HepG2 lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Performs the first committed step in the biosynthesis of isoprenes.
    • Tissue specificity

      Expressed in heart, skeletal muscle, lung, liver, brain, pancreas, kidney and placenta.
    • Pathway

      Steroid biosynthesis; cholesterol biosynthesis.
    • Sequence similarities

      Belongs to the diphosphomevalonate decarboxylase family.
    • Information by UniProt
    • Database links

    • Alternative names

      • Diphosphomevalonate decarboxylase antibody
      • FP17780 antibody
      • MDDase antibody
      • Mevalonate (diphospho) decarboxylase antibody
      • Mevalonate (diphospho)decarboxylase antibody
      • Mevalonate pyrophosphate decarboxylase antibody
      • MPD antibody
      • Mvd antibody
      • MVD1_HUMAN antibody
      see all

    Images

    • All lanes : Anti-MVD antibody [EPR6559(2)] (ab129061) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : K562 cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 43 kDa
      Observed band size: 43 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Zhang P  et al. Intrinsic BET inhibitor resistance in SPOP-mutated prostate cancer is mediated by BET protein stabilization and AKT-mTORC1 activation. Nat Med 23:1055-1062 (2017). Read more (PubMed: 28805822) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HMEC)
    Gel Running Conditions
    Reduced Denaturing (4-12% bis-tris)
    Loading amount
    10000 cells
    Treatment
    MVD shRNAs induced for 72hrs
    Specification
    HMEC
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Jul 28 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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