- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-MVK antibody
See all MVK primary antibodies -
DescriptionRabbit polyclonal to MVK
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment corresponding to a region within amino acids 163-396 of Human MVK (UniProt ID: Q03426).
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Positive control
- 293T, A431, HepG2, Raji whole cell lysate and A549 xenograft.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab154515 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 42 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used. |
Target
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FunctionMay be a regulatory site in cholesterol biosynthetic pathway.
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PathwayIsoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 1/3.
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Involvement in diseaseDefects in MVK are the cause of mevalonic aciduria (MEVA) [MIM:610377]. It is an accumulation of mevalonic acid which causes a variety of symptoms such as psychomotor retardation, dysmorphic features, cataracts, hepatosplenomegaly, lymphadenopathy, anemia, hypotonia, myopathy, and ataxia.
Defects in MVK are the cause of hyperimmunoglobulinemia D and periodic fever syndrome (HIDS) [MIM:260920]. HIDS is an autosomal recessive disease characterized by recurrent episodes of unexplained high fever associated with skin rash, diarrhea, adenopathy (swollen, tender lymph nodes), athralgias and/or arthritis. Concentration of IgD, and often IgA, are above normal. -
Sequence similaritiesBelongs to the GHMP kinase family. Mevalonate kinase subfamily.
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Cellular localizationCytoplasm. Peroxisome.
- Information by UniProt
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Database links
- Entrez Gene: 4598 Human
- Entrez Gene: 17855 Mouse
- Entrez Gene: 81727 Rat
- Omim: 251170 Human
- SwissProt: Q03426 Human
- SwissProt: Q9R008 Mouse
- SwissProt: P17256 Rat
- Unigene: 130607 Human
see all -
Alternative names
- FLJ96772 antibody
- KIME_HUMAN antibody
- LH receptor mRNA binding protein antibody
see all
Images
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Western blot - Anti-MVK antibody (ab154515)Anti-MVK antibody (ab154515) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 42 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVK antibody (ab154515)Immunohistochemical analysis of formalin-fixed, paraffin-embedded A549 xenograft labeling MVK with ab154515 at 1/500.
References
ab154515 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"