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Overview
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Product nameAnti-MVK antibody [EPR6507]
See all MVK primary antibodies -
DescriptionRabbit monoclonal [EPR6507] to MVK
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human MVK aa 300-400. The exact sequence is proprietary.
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Positive control
- Permeabilized HepG2 cells; Human fetal liver, HepG2, Jurkat and Caco-2 cell lysates.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Dissociation constant (KD)KD = 1.88 x 10 -10 M Learn more about KD
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR6507
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab126619 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). | |
| IP | 1/10 - 1/100. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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FunctionMay be a regulatory site in cholesterol biosynthetic pathway.
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PathwayIsoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 1/3.
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Involvement in diseaseDefects in MVK are the cause of mevalonic aciduria (MEVA) [MIM:610377]. It is an accumulation of mevalonic acid which causes a variety of symptoms such as psychomotor retardation, dysmorphic features, cataracts, hepatosplenomegaly, lymphadenopathy, anemia, hypotonia, myopathy, and ataxia.
Defects in MVK are the cause of hyperimmunoglobulinemia D and periodic fever syndrome (HIDS) [MIM:260920]. HIDS is an autosomal recessive disease characterized by recurrent episodes of unexplained high fever associated with skin rash, diarrhea, adenopathy (swollen, tender lymph nodes), athralgias and/or arthritis. Concentration of IgD, and often IgA, are above normal. -
Sequence similaritiesBelongs to the GHMP kinase family. Mevalonate kinase subfamily.
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Cellular localizationCytoplasm. Peroxisome.
- Information by UniProt
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Database links
- Entrez Gene: 4598 Human
- Omim: 251170 Human
- SwissProt: Q03426 Human
- Unigene: 130607 Human
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Alternative names
- FLJ96772 antibody
- KIME_HUMAN antibody
- LH receptor mRNA binding protein antibody
see all
Images
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![Western blot - Anti-MVK antibody [EPR6507] (ab126619)](https://www.abcam.com/ps/products/126/ab126619/Images/MVK-Primary-antibodies-ab126619-1.jpg)
Western blot - Anti-MVK antibody [EPR6507] (ab126619)All lanes : Anti-MVK antibody [EPR6507] (ab126619) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Caco-2 cell lysate
Lane 4 : Human fetal liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa -
![Flow Cytometry - Anti-MVK antibody [EPR6507] (ab126619)](https://www.abcam.com/ps/products/126/ab126619/Images/MVK-Primary-antibodies-ab126619-2.jpg)
Flow Cytometry - Anti-MVK antibody [EPR6507] (ab126619)ab126619, at a 1/10 dilution, positively staining MVK in permeabilized HepG2 cells (red). A Rabbit IgG was used as negative control (green). -
![Other - Anti-MVK antibody [EPR6507] (ab126619)](https://www.abcam.com/ps/products/126/ab126619/Images/MVK-Primary-antibodies-ab126619-3.jpg)
Other - Anti-MVK antibody [EPR6507] (ab126619)
References
ab126619 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"