Recombinant Anti-MVK antibody [EPR6507] (ab126619)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-MVK antibody [EPR6507]
See all MVK primary antibodies -
Description
Rabbit monoclonal [EPR6507] to MVK -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human MVK aa 300-400. The exact sequence is proprietary.
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Positive control
- Permeabilized HepG2 cells; Human fetal liver, HepG2, Jurkat and Caco-2 cell lysates.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.88 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6507 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab126619 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). | |
| IP | 1/10 - 1/100. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
Target
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Function
May be a regulatory site in cholesterol biosynthetic pathway. -
Pathway
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 1/3. -
Involvement in disease
Defects in MVK are the cause of mevalonic aciduria (MEVA) [MIM:610377]. It is an accumulation of mevalonic acid which causes a variety of symptoms such as psychomotor retardation, dysmorphic features, cataracts, hepatosplenomegaly, lymphadenopathy, anemia, hypotonia, myopathy, and ataxia.
Defects in MVK are the cause of hyperimmunoglobulinemia D and periodic fever syndrome (HIDS) [MIM:260920]. HIDS is an autosomal recessive disease characterized by recurrent episodes of unexplained high fever associated with skin rash, diarrhea, adenopathy (swollen, tender lymph nodes), athralgias and/or arthritis. Concentration of IgD, and often IgA, are above normal. -
Sequence similarities
Belongs to the GHMP kinase family. Mevalonate kinase subfamily. -
Cellular localization
Cytoplasm. Peroxisome. - Information by UniProt
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Database links
- Entrez Gene: 4598 Human
- Omim: 251170 Human
- SwissProt: Q03426 Human
- Unigene: 130607 Human
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Alternative names
- FLJ96772 antibody
- KIME_HUMAN antibody
- LH receptor mRNA binding protein antibody
see all
Images
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![Western blot - Anti-MVK antibody [EPR6507] (ab126619)](https://www.abcam.com/ps/products/126/ab126619/Images/ab126619-140611-anti-mvk-antibody-epr6507-western-blot.jpg)
Western blot - Anti-MVK antibody [EPR6507] (ab126619)All lanes : Anti-MVK antibody [EPR6507] (ab126619) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Caco-2 cell lysate
Lane 4 : Human fetal liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa -
![Flow Cytometry - Anti-MVK antibody [EPR6507] (ab126619)](https://www.abcam.com/ps/products/126/ab126619/Images/ab126619-140610-anti-mvk-antibody-epr6507-flow-cytometry.jpg)
Flow Cytometry - Anti-MVK antibody [EPR6507] (ab126619)ab126619, at a 1/10 dilution, positively staining MVK in permeabilized HepG2 cells (red). A Rabbit IgG was used as negative control (green). -
![Other - Anti-MVK antibody [EPR6507] (ab126619)](https://www.abcam.com/ps/products/126/ab126619/Images/ab126619-140609-anti-mvk-antibody-epr6507.jpg)
Other - Anti-MVK antibody [EPR6507] (ab126619)
Datasheets and documents
References
ab126619 has not yet been referenced specifically in any publications.
