Overview

  • Product name

    Anti-MVP antibody [1032] - BSA and Azide free
    See all MVP primary antibodies
  • Description

    Mouse monoclonal [1032] to MVP - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human MVP. (Proteins precipitated from Human breast cancer MCF7 cells).

  • Positive control

    • Human testicular carcinoma and breast carcinoma tissues; MCF7 and HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: 100% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    ab213122 was purified from bioreactor concentrate by Protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    1032
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab213122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.5-1µg for 106 cells.

(in 0.1 ml).

IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

(Primary incubation for 30 minutes at RT).

ICC/IF Use a concentration of 0.5 - 1 µg/ml.

Target

  • Function

    Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.
  • Tissue specificity

    Present in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells.
  • Sequence similarities

    Contains 9 MVP (vault) repeats.
  • Domain

    MVP 3 mediates interaction with PTEN.
    MVP 4 mediates interaction with PARP4.
  • Post-translational
    modifications

    Phosphorylated on Tyr residues after EGF stimulation.
    Dephosphorylated by PTPN11.
  • Cellular localization

    Cytoplasm. Nucleus > nuclear pore complex. 5% found in the nuclear pore complex. Translocates from the nucleus to the cytoplasm upon EGF treatment.
  • Information by UniProt
  • Database links

  • Alternative names

    • LRP antibody
    • Lung resistance related protein antibody
    • Lung resistance-related protein antibody
    • Major vault protein antibody
    • Major vault protein, rat, homolog of antibody
    • MVP antibody
    • MVP_HUMAN antibody
    • testicular secretory protein Li 30 antibody
    • VAULT 1 antibody
    • VAULT1 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testicular carcinoma tissue labeling MVP with ab213122 at 1 µg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labeling MVP with ab213122 at 1 µg/ml.

References

ab213122 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab213122.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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