Overview

  • Product name

  • Description

    Rabbit polyclonal to MVP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Cat
  • Immunogen

    Recombinant fragment: EVV EIIQATIIRQ NQALRLRARK ECWDRDGKER VTGEEWLVTT VGAYLPAVFE EVLDLVDAVI LTEKTALHLR ARRNFRDFRG VSRRTGEEWL VTVQDTEAHV PDVHEEVLGV VPITTLGPHN YCVILDPVGP DGKNQLGQKR VVKGEKSFFL QPGEQLEQGI QDVYVLSEQQ GLLLRALQPL EEGEDEEKVS, corresponding to amino acids 158-350 of Human MVP (AAH15623)

  • Positive control

    • 293T, A431, H1299, HeLa, HepG2, MOLT4, A549 and Raji cells

Properties

Applications

Our Abpromise guarantee covers the use of ab97311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 22125622
WB 1/500 - 1/3000. Predicted molecular weight: 99 kDa.
IHC-P 1/100 - 1/500.

Target

  • Function

    Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.
  • Tissue specificity

    Present in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells.
  • Sequence similarities

    Contains 9 MVP (vault) repeats.
  • Domain

    MVP 3 mediates interaction with PTEN.
    MVP 4 mediates interaction with PARP4.
  • Post-translational
    modifications

    Phosphorylated on Tyr residues after EGF stimulation.
    Dephosphorylated by PTPN11.
  • Cellular localization

    Cytoplasm. Nucleus > nuclear pore complex. 5% found in the nuclear pore complex. Translocates from the nucleus to the cytoplasm upon EGF treatment.
  • Information by UniProt
  • Database links

  • Alternative names

    • LRP antibody
    • Lung resistance related protein antibody
    • Lung resistance-related protein antibody
    • Major vault protein antibody
    • Major vault protein, rat, homolog of antibody
    • MVP antibody
    • MVP_HUMAN antibody
    • testicular secretory protein Li 30 antibody
    • VAULT 1 antibody
    • VAULT1 antibody
    see all

Images

  • All lanes : Anti-MVP antibody (ab97311) at 1/500 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : MVP Knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 99 kDa

  • Anti-MVP antibody (ab97311) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size: 99 kDa



    7.5% SDS-PAGE
  • Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ab97311 at 1/500 dilution.

References

This product has been referenced in:

  • Shi L  et al. Up-regulation of miR-146a increases the sensitivity of non-small cell lung cancer to DDP by downregulating cyclin J. BMC Cancer 17:138 (2017). WB . Read more (PubMed: 28202053) »
  • O'Dwyer D  et al. The proteomics of colorectal cancer: identification of a protein signature associated with prognosis. PLoS One 6:e27718 (2011). IHC-Fr ; Human . Read more (PubMed: 22125622) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Skeletal Muscle)
Loading amount
30 µg
Specification
Skeletal Muscle
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jun 30 2012

Answer

DISCOUNT CODE: ***********
Expiration date: *****************
I am very pleased to hear you would like to accept our offer and test ab97311 in mouse tissue. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for mouse tissue and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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