Recombinant
RabMAb

Recombinant Anti-MVP antibody [EPR13226(B)] (ab177145)

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Overview

  • Product name

    Anti-MVP antibody [EPR13226(B)]
    See all MVP primary antibodies

  • Description

    Rabbit monoclonal [EPR13226(B)] to MVP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human MVP aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q14764

  • Positive control

    • Human spleen, Calu3 and A549 lysates.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR13226(B)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab177145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.

    • Target

    • Function

      Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.

    • Tissue specificity

      Present in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells.

    • Sequence similarities

      Contains 9 MVP (vault) repeats.

    • Domain

      MVP 3 mediates interaction with PTEN.
      MVP 4 mediates interaction with PARP4.

    • Post-translational
      modifications

      Phosphorylated on Tyr residues after EGF stimulation.
      Dephosphorylated by PTPN11.

    • Cellular localization

      Cytoplasm. Nucleus > nuclear pore complex. 5% found in the nuclear pore complex. Translocates from the nucleus to the cytoplasm upon EGF treatment.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • LRP antibody
      • Lung resistance related protein antibody
      • Lung resistance-related protein antibody
      • Major vault protein antibody
      • Major vault protein, rat, homolog of antibody
      • MVP antibody
      • MVP_HUMAN antibody
      • testicular secretory protein Li 30 antibody
      • VAULT 1 antibody
      • VAULT1 antibody
      see all

    Images

    • Western blot - Anti-MVP antibody [EPR13226(B)] (ab177145)
      Western blot - Anti-MVP antibody [EPR13226(B)] (ab177145)
      All lanes : Anti-MVP antibody [EPR13226(B)] (ab177145) at 1/1000 dilution

      Lane 1 : Human spleen lysate
      Lane 2 : Calu3 lysate
      Lane 3 : A549 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 99 kDa

    References

    ab177145 has not yet been referenced specifically in any publications.

    Publishing research using ab177145? Please let us know so that we can cite the reference in this datasheet.

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