Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-MVP antibody [EPR13226(B)] (ab177145)

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Western blot - Anti-MVP antibody [EPR13226(B)] (ab177145)
  • Western blot - Anti-MVP antibody [EPR13226(B)] (ab177145)
  • Anti-MVP antibody [EPR13226(B)] (ab177145)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13226(B)] to MVP
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MVP antibody [EPR13226(B)]
    See all MVP primary antibodies

  • Description

    Rabbit monoclonal [EPR13226(B)] to MVP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human MVP aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q14764

  • Positive control

    • Human spleen, Calu3 and A549 lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR13226(B)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab177145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.

    • Target

    • Function

      Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.

    • Tissue specificity

      Present in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells.

    • Sequence similarities

      Contains 9 MVP (vault) repeats.

    • Domain

      MVP 3 mediates interaction with PTEN.
      MVP 4 mediates interaction with PARP4.

    • Post-translational
      modifications

      Phosphorylated on Tyr residues after EGF stimulation.
      Dephosphorylated by PTPN11.

    • Cellular localization

      Cytoplasm. Nucleus > nuclear pore complex. 5% found in the nuclear pore complex. Translocates from the nucleus to the cytoplasm upon EGF treatment.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • LRP antibody
      • Lung resistance related protein antibody
      • Lung resistance-related protein antibody
      • Major vault protein antibody
      • Major vault protein, rat, homolog of antibody
      • MVP antibody
      • MVP_HUMAN antibody
      • testicular secretory protein Li 30 antibody
      • VAULT 1 antibody
      • VAULT1 antibody
      see all

    Images

    • Western blot - Anti-MVP antibody [EPR13226(B)] (ab177145)
      Western blot - Anti-MVP antibody [EPR13226(B)] (ab177145)
      All lanes : Anti-MVP antibody [EPR13226(B)] (ab177145) at 1/1000 dilution

      Lane 1 : Wild-type HeLa lysate
      Lane 2 : MVP knockout HeLa lysate
      Lane 3 : A549 lysate
      Lane 4 : MOLT-4 lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 99 kDa



      Lanes 1-4: Merged signal (red and green). Green - ab177145 observed at 99 kDa. Red - loading control ab8245 observed at 37 kDa.

       ab177145 Anti-MVP antibody [EPR13226(B)] was shown to specifically react with MVP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264817 (knockout cell lysate ab257544) was used. Wild-type and MVP knockout samples were subjected to SDS-PAGE. ab177145 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

       

    • Western blot - Anti-MVP antibody [EPR13226(B)] (ab177145)
      Western blot - Anti-MVP antibody [EPR13226(B)] (ab177145)
      All lanes : Anti-MVP antibody [EPR13226(B)] (ab177145) at 1/1000 dilution

      Lane 1 : Human spleen lysate
      Lane 2 : Calu3 lysate
      Lane 3 : A549 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 99 kDa

    • Anti-MVP antibody [EPR13226(B)] (ab177145)
      Anti-MVP antibody [EPR13226(B)] (ab177145)

    References (0)

    Publishing research using ab177145? Please let us know so that we can cite the reference in this datasheet.

    ab177145 has not yet been referenced specifically in any publications.

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