Anti-MVP antibody [EPR13226(B)] (ab177145)
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- Specific References
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Overview
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Product nameAnti-MVP antibody [EPR13226(B)]
See all MVP primary antibodies -
DescriptionRabbit monoclonal [EPR13226(B)] to MVP
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human MVP aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q14764 -
Positive control
- Human spleen, Calu3 and A549 lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR13226(B)
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab177145 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa). | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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FunctionRequired for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.
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Tissue specificityPresent in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells.
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Sequence similaritiesContains 9 MVP (vault) repeats.
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DomainMVP 3 mediates interaction with PTEN.
MVP 4 mediates interaction with PARP4. -
Post-translational
modificationsPhosphorylated on Tyr residues after EGF stimulation.
Dephosphorylated by PTPN11. -
Cellular localizationCytoplasm. Nucleus > nuclear pore complex. 5% found in the nuclear pore complex. Translocates from the nucleus to the cytoplasm upon EGF treatment.
- Information by UniProt
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Database links
- Entrez Gene: 9961 Human
- Entrez Gene: 78388 Mouse
- Entrez Gene: 64681 Rat
- Omim: 605088 Human
- SwissProt: Q14764 Human
- SwissProt: Q9EQK5 Mouse
- SwissProt: Q62667 Rat
- Unigene: 632177 Human
see all -
Alternative names
- LRP antibody
- Lung resistance related protein antibody
- Lung resistance-related protein antibody
see all
Images
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![Western blot - Anti-MVP antibody [EPR13226(B)] (ab177145)](https://www.abcam.com/ps/products/177/ab177145/Images/ab177145-205112-ab177145.jpg)
Western blot - Anti-MVP antibody [EPR13226(B)] (ab177145)All lanes : Anti-MVP antibody [EPR13226(B)] (ab177145) at 1/1000 dilution
Lane 1 : Human spleen lysate
Lane 2 : Calu3 lysate
Lane 3 : A549 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 99 kDa
References
ab177145 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"