Name: Anti-MVP antibody [EPR13227(B)]
Brand: RabMAb
SKU: ab175239

Product name

Anti-MVP antibody [EPR13227(B)]
See all MVP primary antibodies
Description

Rabbit monoclonal [EPR13227(B)] to MVP
Host species

Rabbit
Tested applications

Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MVP aa 850 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q14764
Positive control
- WB: A549, Calu-3,Caco-2 and HeLa whole cell lysate (ab150035). IHC-P: Human colon and human pancreas tissues. ICC/IF: A549 and HeLa cells. Flow Cyt: A549 cells.
General notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR13227(B)
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab175239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/2000. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa). For unpurified use at 1/10000 - 1/50000.
ICC/IF		1/150 - 1/500.
Flow Cyt		1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P		1/50 - 1/350. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols.

Function

Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.
Tissue specificity

Present in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells.
Sequence similarities

Contains 9 MVP (vault) repeats.
Domain

MVP 3 mediates interaction with PTEN.
MVP 4 mediates interaction with PARP4.
Post-translational
modifications

Phosphorylated on Tyr residues after EGF stimulation.
Dephosphorylated by PTPN11.
Cellular localization

Cytoplasm. Nucleus > nuclear pore complex. 5% found in the nuclear pore complex. Translocates from the nucleus to the cytoplasm upon EGF treatment.
Target information above from: UniProt accession Q14764 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 9961 Human
- Omim: 605088 Human
- SwissProt: Q14764 Human
- Unigene: 632177 Human
Alternative names
- LRP antibody
- Lung resistance related protein antibody
- Lung resistance-related protein antibody
- Major vault protein antibody
- Major vault protein, rat, homolog of antibody
- MVP antibody
- MVP_HUMAN antibody
- testicular secretory protein Li 30 antibody
- VAULT 1 antibody
- VAULT1 antibody
see all

Western blot - Anti-MVP antibody [EPR13227(B)] (ab175239)

Anti-MVP antibody [EPR13227(B)] (ab175239) at 1/2000 dilution (purified) + Caco-2 whole cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 99 kDa
Observed band size: 110 kDa
why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST
Western blot - Anti-MVP antibody [EPR13227(B)] (ab175239)

All lanes : Anti-MVP antibody [EPR13227(B)] (ab175239) at 1/10000 dilution (purified)

Lane 1 : Calu-3 whole cell lysate
Lane 2 : A549 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 99 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR13227(B)] (ab175239)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling MVP with purified ab175239 at a dilution of 1/350. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunocytochemistry/ Immunofluorescence - Anti-MVP antibody [EPR13227(B)] (ab175239)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling MVP with purified ab175239 at a dilution of 1/150. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

Control 1: primary antibody (1/150) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000).

Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000).
Flow Cytometry - Anti-MVP antibody [EPR13227(B)] (ab175239)

Flow Cytometry analysis of A549 cells labelling MVP with purified ab175239 at a dilution of 1/180 (red). Cells were fixed with 4% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
Western blot - Anti-MVP antibody [EPR13227(B)] (ab175239)

All lanes : Anti-MVP antibody [EPR13227(B)] (ab175239) at 1/10000 dilution (unpurified)

Lane 1 : A549 cell lysate
Lane 2 : Calu-3 cell lysate
Lane 3 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 99 kDa
Additional bands at: 110 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR13227(B)] (ab175239)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labeling MVP with unpurified ab175239 at a dilution of 1/50.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR13227(B)] (ab175239)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labeling MVP with unpurified ab175239 at a dilution of 1/50.
Immunocytochemistry/ Immunofluorescence - Anti-MVP antibody [EPR13227(B)] (ab175239)

Immunocytochemistry/Immunofluorescence analysis of A549 cells labeling MVP with unpurified ab175239 at a dilution of 1/250 (red) and DAPI staining (blue).
Flow Cytometry - Anti-MVP antibody [EPR13227(B)] (ab175239)

Flow Cytometrical analysis of permeabilized A549 cells labeling MVP with unpurified ab175239 antibody at a dilution of 1/100 (red) compared to a negative control (Rabbit IgG, green).

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Datasheet

This product has been referenced in:

Galbiati E et al. A fast and straightforward procedure for vault nanoparticle purification and the characterization of its endocytic uptake. Biochim Biophys Acta Gen Subj 1862:2254-2260 (2018). Read more (PubMed: 30036602) »
Wang S et al. Effect and mechanism of resveratrol on drug resistance in human bladder cancer cells. Mol Med Rep 15:1179-1187 (2017). Read more (PubMed: 28098863) »

See all 2 Publications for this product

Publishing research using ab175239? Please let us know so that we can cite the reference in this datasheet.

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Anti-MVP antibody [EPR13227(B)] (ab175239)

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