Overview

  • Product name
    Anti-MVP antibody [LMR5]
    See all MVP primary antibodies
  • Description
    Rat monoclonal [LMR5] to MVP
  • Host species
    Rat
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation; 2R120 cell line, derived by stepwise Doxorubicin exposure of the SW-1573 non-small cell lung carcinoma cell line.

  • Positive control
    • Positive tissue - Cell Lines: Drug-sensitive parental cell lines and their multidrugresistant derivatives. Negative control antibody: Normal rat serum.

Properties

Applications

Our Abpromise guarantee covers the use of ab3374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/20 - 1/30.

Cell permeabilization required.

ab37371 - Rat polyclonal IgG, is suitable for use as an isotype control with this antibody. 

IHC-P 1/20 - 1/30.
IHC-Fr 1/20 - 1/30.

Target

  • Function
    Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.
  • Tissue specificity
    Present in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells.
  • Sequence similarities
    Contains 9 MVP (vault) repeats.
  • Domain
    MVP 3 mediates interaction with PTEN.
    MVP 4 mediates interaction with PARP4.
  • Post-translational
    modifications
    Phosphorylated on Tyr residues after EGF stimulation.
    Dephosphorylated by PTPN11.
  • Cellular localization
    Cytoplasm. Nucleus > nuclear pore complex. 5% found in the nuclear pore complex. Translocates from the nucleus to the cytoplasm upon EGF treatment.
  • Information by UniProt
  • Database links
  • Alternative names
    • LRP antibody
    • Lung resistance related protein antibody
    • Lung resistance-related protein antibody
    • Major vault protein antibody
    • Major vault protein, rat, homolog of antibody
    • MVP antibody
    • MVP_HUMAN antibody
    • testicular secretory protein Li 30 antibody
    • VAULT 1 antibody
    • VAULT1 antibody
    see all

References

This product has been referenced in:
  • Schroeijers AB  et al. Immunohistochemical detection of the human major vault protein LRP with two monoclonal antibodies in formalin-fixed, paraffin-embedded tissues. Am J Pathol 152:373-8 (1998). Read more (PubMed: 9466563) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for contacting us.

The Anti-MVP antibody [LMR5] (ab3374) is provided in the form of tissue culture supernatant. The exact concentration of the antibody present in the sample is therefore difficult to determine due to the other proteins also present. Typically you would find the concentration to be approximately 1-3 mg/mL. I am sorry that the information on the datasheet has been confusing, we are working on trying to remedy this.

I hope this information has been of help. If you require any further information please do not hesitate to ask.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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