Key features and details
- Rabbit polyclonal to MVP - N-terminal
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MVP antibody - N-terminal
See all MVP primary antibodies
DescriptionRabbit polyclonal to MVP - N-terminal
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
Synthetic peptide within Human MVP aa 1-50 (N terminal). The exact sequence is proprietary.
Database link: Q14764
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human colon carcinoma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226464 was affinity purified using an epitope specific to MVP Vault Protein immobilized on solid support.
Our Abpromise guarantee covers the use of ab226464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 99 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionRequired for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.
Tissue specificityPresent in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells.
Sequence similaritiesContains 9 MVP (vault) repeats.
DomainMVP 3 mediates interaction with PTEN.
MVP 4 mediates interaction with PARP4.
modificationsPhosphorylated on Tyr residues after EGF stimulation.
Dephosphorylated by PTPN11.
Cellular localizationCytoplasm. Nucleus > nuclear pore complex. 5% found in the nuclear pore complex. Translocates from the nucleus to the cytoplasm upon EGF treatment.
- Information by UniProt
- LRP antibody
- Lung resistance related protein antibody
- Lung resistance-related protein antibody
All lanes : Anti-MVP antibody - N-terminal (ab226464) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 99 kDa
Exposure time: 3 minutes
Cells prepared using NETN lysis buffer.
Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for MVP using ab226464 at 1/1000 dilution in immunohistochemical analysis.
Detection: DAB staining.
MVP was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226464 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226464 at 1 µg/ml.
Lane 1: ab226464 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Cells prepared using NETN lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226464 has not yet been referenced specifically in any publications.