Key features and details
- Rabbit polyclonal to MX1
- Suitable for: IHC-P, ICC/IF, IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-MX1 antibody
See all MX1 primary antibodies
DescriptionRabbit polyclonal to MX1
Tested applicationsSuitable for: IHC-P, ICC/IF, IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rhesus monkey, Orangutan
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab222856 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
|IP||1/200 - 1/2000.|
|WB||1/1000 - 1/5000. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).|
FunctionMay regulate the calcium channel activity of TRPCs. Shows activity against influenza virus and VSV, a rhabdovirus.
Sequence similaritiesBelongs to the dynamin family.
Contains 1 GED domain.
- Information by UniProt
- Human interferon regulated resistance GTP binding protein MXA antibody
- IFI 78 antibody
- IFI 78K antibody
All lanes : Anti-MX1 antibody (ab222856) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Predicted band size: 76 kDa
Observed band size: 76 kDa
Paraffin-embedded human pancreatic tissue stained for MX1 using ab222856 at 1/100 dilution in immunohistochemical analysis.
PC-3 (human prostate adenocarcinoma cell line) cells stained for MX1 (green) using ab222856 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).
MX1 was immunoprecipitated from 500 µg HeLa (human epithelial cell line from cervix adenocarcinomaline) whole cell lysate with 4 µg ab222856. Western blot was performed from the immunoprecipitate using ab222856.
Lane 1: Control IgG IP in HeLa whole cell lysate.
Lane 2: ab222856 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate (20 µg).
For western blotting, an HRP-conjugated light chain specific antibody was used as the secondary antibody at 1/50000 dilution.
ab222856 has not yet been referenced specifically in any publications.