Product nameMXI1 overexpression 293T lysate (whole cell)
ab94151 is a 293T cell transfected lysate in which Human MXI1 has been transiently over-expressed using a pCMV-MXI1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundFunction: Transcriptional repressor. MXI1 binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. MXI1 thus antagonizes MYC transcriptional activity by competing for MAX. Tissue specificity: High levels found in the brain, heart and lung while lower levels are seen in the liver, kidney and skeletal muscle. Disease: Defects in MXI1 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma. Similarity: Contains 1 basic helix-loop-helix (bHLH) domain.
Our Abpromise guarantee covers the use of ab94151 in the following tested applications.
|WB||Use at an assay dependent dilution.|
ab94151 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-MXI1 antibody (ab76881) at 1/500 dilution
Lane 1 :
MXI1 overexpression 293T lysate (whole cell) (ab94151)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94151 has not yet been referenced specifically in any publications.