Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7204] to MYBBP1A
- Suitable for: WB, IHC-P, ICC, Flow Cyt
- Reacts with: Human
Product nameAnti-MYBBP1A antibody [EPR7204]
See all MYBBP1A primary antibodies
DescriptionRabbit monoclonal [EPR7204] to MYBBP1A
Tested applicationsSuitable for: WB, IHC-P, ICC, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide within Human MYBBP1A aa 1-100. The exact sequence is proprietary.
- HeLa, TF1, and Jurkat cell lysates; Human kidney tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab125026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 148 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC||1/100 - 1/250.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionMay activate or repress transcription via interactions with sequence specific DNA-binding proteins. Repression may be mediated at least in part by histone deacetylase activity (HDAC activity).
Cellular localizationCytoplasm. Nucleus. Nucleus > nucleolus. Shuttles between the nucleus and cytoplasm. Nuclear import may be mediated by KPNA2, while export appears to depend partially on XPO1/CRM1 (By similarity). Predominantly nucleolar.
- Information by UniProt
- AL024407 antibody
- AU019902 antibody
- cb486 antibody
All lanes : Anti-MYBBP1A antibody [EPR7204] (ab125026) at 1/1000 dilution
Lane 1 : Hela cell lysates
Lane 2 : TF1 cell lysates
Lane 3 : Jurkat cell lysates
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 148 kDa
ab125026 at 1/100 dilution, staining MYBBP1A in Paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab125026 has not yet been referenced specifically in any publications.