Overview

  • Product name

  • Description

    Rabbit polyclonal to MYBPC1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal sequence of Human MYBPC1.

  • Positive control

    • 293 cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab110363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 128 kDa.

Target

  • Function

    Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily. MyBP family.
    Contains 3 fibronectin type-III domains.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
  • Information by UniProt
  • Database links

  • Alternative names

    • C protein, skeletal muscle slow isoform antibody
    • C-protein antibody
    • MYBPC1 antibody
    • MYBPCC antibody
    • MYBPCS antibody
    • Myosin binding protein C, slow type antibody
    • Myosin-binding protein C antibody
    • MYPC1_HUMAN antibody
    • skeletal muscle C protein antibody
    • skeletal muscle slow isoform antibody
    • Slow MyBP C antibody
    • Slow MyBP-C antibody
    • slow-type antibody
    see all

Images

  • All lanes : Anti-MYBPC1 antibody (ab110363) at 1/500 dilution

    Lane 1 : 293 cell extract
    Lane 2 : 293 cell extract with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 128 kDa

References

ab110363 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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1-4 of 4 Abreviews

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart)
Permeabilization
Yes - Tween-20
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 22 2016

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (4%-12%)
Sample
Mouse Tissue lysate - whole (Skeletal Muscle)
Specification
Skeletal Muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 31 2014

Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Non-reduced Denaturing (10%)
Sample
Mouse Tissue lysate - whole (Skeletal Muscle)
Specification
Skeletal Muscle
Blocking step
WB Blocking Solution (commercial) as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C

Mr. Brian Lin

Verified customer

Submitted Jul 23 2014

Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Rat Tissue lysate - whole (skeletal muscle)
Specification
skeletal muscle
Blocking step
WB blocking buffer as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C

Mr. Brian Lin

Verified customer

Submitted Jul 14 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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