Western blot abreview for Anti-MYBPC1 antibody

Average
Abreviews
Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Non-reduced Denaturing (10%)
Sample
Mouse Tissue lysate - whole (Skeletal Muscle)
Specification
Skeletal Muscle
Blocking step
WB Blocking Solution (commercial) as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C

Other product details

Incubation time
15 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 10% WB Blocking Solution
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: goat anti-rabbit IgG-HRP: sc-2004
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL
Bands
Specific: 150 kDa Non-specific: 100 kDa
Exposure
1 minute(s) and 0 second(s)
Negative control
Marker Lane
Positive control
Skeletal Muscles were previously probed for slow MyBP-C excised from mouse tissues: EDL (extensor digitorum longus), FDB (flexor digitorum brevis), soleus, and TA (tibialis anterior)

Additional data

Additional Notes
Single Band: 140 kDa - 100 kDa, Reactivity to marker (Biorad Precision Plus Prestained Ladder) Legend: Lane 1 = m = marker Lane 2 = EDL = extensor digitorum longus Lane 3 = FDB = flexor digitorum brevis Lane 4 = SOL = soleus Lane 5 = TA = tibialis anterior

Mr. Brian Lin

Verified customer

Submitted Jul 23 2014

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