Western blot abreview for Anti-MYBPC1 antibody

Average
Abreviews
Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (4%-12%)
Sample
Mouse Tissue lysate - whole (Skeletal Muscle)
Specification
Skeletal Muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBST with 2.5%BSA
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Polyclonal Goat Anti Rabbit Immunoglobulins/HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL
Bands
Specific: 140 kDa Non-specific: 80, 95, 100 kDa
Exposure
2 minute(s) and 0 second(s)
Positive control
MYBPC1 is a protein highly expressed in skeletal muscle.

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Submitted Dec 31 2014

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