Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3008(2)] to MYBPC3 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Product nameAnti-MYBPC3 antibody [EPR3008(2)] - BSA and Azide free
See all MYBPC3 primary antibodies
DescriptionRabbit monoclonal [EPR3008(2)] to MYBPC3 - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Human
Synthetic peptide within Human MYBPC3 aa 100-200. The exact sequence is proprietary.
- Human heart lysate
ab203258 is the carrier-free version of ab108522 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab203258 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab203258 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 141 kDa.|
FunctionThick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role.
Involvement in diseaseDefects in MYBPC3 are the cause of cardiomyopathy familial hypertrophic type 4 (CMH4) [MIM:115197]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Sequence similaritiesBelongs to the immunoglobulin superfamily. MyBP family.
Contains 3 fibronectin type-III domains.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
modificationsSubstrate for phosphorylation by PKA and PKC. Reversible phosphorylation appears to modulate contraction.
- Information by UniProt
- C protein cardiac muscle isoform antibody
- C-protein antibody
- cardiac muscle isoform antibody
Anti-MYBPC3 antibody [EPR3008(2)] (ab108522) at 1/5000 dilution (purified) + Human heart tissue lysate at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 141 kDa
Observed band size: 141 kDa
This data was developed using ab108522, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab203258 has not yet been referenced specifically in any publications.