Recombinant
RabMAb

Recombinant Anti-MYBPC3 antibody [EPR3009(2)] (ab133499)

Overview

  • Product name

    Anti-MYBPC3 antibody [EPR3009(2)]
    See all MYBPC3 primary antibodies
  • Description

    Rabbit monoclonal [EPR3009(2)] to MYBPC3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human MYBPC3 aa 1200-1300 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Human fetal heart, Rat heart, and Mouse heart lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 141 kDa).
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role.
    • Involvement in disease

      Defects in MYBPC3 are the cause of cardiomyopathy familial hypertrophic type 4 (CMH4) [MIM:115197]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    • Sequence similarities

      Belongs to the immunoglobulin superfamily. MyBP family.
      Contains 3 fibronectin type-III domains.
      Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    • Post-translational
      modifications

      Substrate for phosphorylation by PKA and PKC. Reversible phosphorylation appears to modulate contraction.
    • Information by UniProt
    • Database links

    • Alternative names

      • C protein cardiac muscle isoform antibody
      • C-protein antibody
      • cardiac muscle isoform antibody
      • Cardiac MyBP C antibody
      • Cardiac MyBP-C antibody
      • Cardiac myosin binding protein C antibody
      • cardiac-type antibody
      • CMH4 antibody
      • FHC antibody
      • MYBP C antibody
      • MYBPC antibody
      • MYBPC3 antibody
      • Myosin binding protein C cardiac antibody
      • Myosin binding protein C cardiac-type antibody
      • Myosin-binding protein C antibody
      • myosin-binding protein C cardiac type antibody
      • MYPC3_HUMAN antibody
      see all

    Images

    • All lanes : Anti-MYBPC3 antibody [EPR3009(2)] (ab133499) at 1/1000 dilution

      Lane 1 : Human fetal heart lysates with 5% NFDM/TBST
      Lane 2 : Mouse heart lysates with 5% NFDM/TBST
      Lane 3 : Rat heart lysate with 5% NFDM/TBST

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 141 kDa
      Observed band size: 150 kDa
      why is the actual band size different from the predicted?

    • All lanes : Anti-MYBPC3 antibody [EPR3009(2)] (ab133499) at 1/1000 dilution

      Lane 1 : Human heart lysate
      Lane 2 : Rat heart lysate
      Lane 3 : Mouse heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 141 kDa
      Observed band size: 150 kDa why is the actual band size different from the predicted?

    References

    ab133499 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Question
    Answer


    I am very pleased to hear you would like to accept our offer and test ab88608 and ab133499in IHC. This code will give you: 1 freePRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an Abreview forIHCand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

    For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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