Anti-Myc tag antibody [9E10] (DyLight® 488) (ab117499)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-Myc tag antibody [9E10] (DyLight® 488)
See all Myc tag primary antibodies -
Description
Mouse monoclonal [9E10] to Myc tag (DyLight® 488) -
Host species
Mouse -
Conjugation
DyLight® 488. Ex: 493nm, Em: 518nm -
Specificity
This antibody is specific for Myc tagged proteins. The Myc tag epitope (EQKLISEEDL) is located at the dimerization site of c-myc and therefore this antibody does not perform well at recognizing endogenous c-myc. -
Tested applications
Suitable for: ICC/IFmore details -
Immunogen
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 8.20
Constituent: 99% Tris buffered saline -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
9E10 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- Anti-Myc tag antibody [9E10] (DyLight® 650) (ab117487)
- Anti-Myc tag antibody [9E10] (DyLight® 550) (ab117511)
- Anti-Myc tag antibody [9E10] (FITC) (ab117599)
- Anti-Myc tag antibody [9E10] - C-terminal (Biotin) (ab197139)
- Anti-Myc tag antibody [9E10] (Alexa Fluor® 488) (ab202008)
- Anti-Myc tag antibody [9E10] (ab206486)
- Anti-Myc tag antibody [9E10] (Alexa Fluor® 594) (ab223894)
- Anti-Myc tag antibody [9E10] (Alexa Fluor® 647) (ab223895)
- Anti-Myc tag antibody [9E10] (Allophycocyanin) (ab223896)
- Anti-Myc tag antibody [9E10] - BSA and Azide free (ab264603)
- Anti-Myc tag antibody [9E10] - ChIP Grade (ab32)
- Anti-Myc tag antibody [9E10] (HRP) (ab62928)
- Anti-Myc tag antibody [9E10] (Phycoerythrin) (ab72468)
- Anti-Myc tag antibody [9E10] (SureLight® Allophycocyanin) (ab72580)
- Anti-Myc tag antibody [9E10] (Biotin) (ab81658)
Applications
Our Abpromise guarantee covers the use of ab117499 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | 1/500 - 1/5000. |
Target
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Relevance
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. -
Cellular localization
Nuclear -
Alternative names
- c-myc tag antibody
- Myc Epitope Tag antibody
Datasheets and documents
References
This product has been referenced in:
- Patankar HV et al. Overexpression of a Metallothionein 2A Gene from Date Palm Confers Abiotic Stress Tolerance to Yeast and Arabidopsis thaliana. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 31212812) »