Overview

  • Product name

    Anti-Myc tag antibody [9E10] (FITC)
    See all Myc tag primary antibodies
  • Description

    Mouse monoclonal [9E10] to Myc tag (FITC)
  • Host species

    Mouse
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Specificity

    This antibody is specific for Myc tagged proteins. The Myc tag epitope (EQKLISEEDL) is located at the dimerization site of c-myc and therefore this antibody does not perform well at recognizing endogenous c-myc.
  • Tested applications

    Suitable for: Flow Cytmore details
  • Immunogen

    Synthetic peptide:

    AEEQKLISEEDLL

    , corresponding to C terminal amino acids 408 - 420 of Human c-Myc

Properties

Applications

Our Abpromise guarantee covers the use of ab117599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10.

Membrane permeabilisation is required for this application. Use 10µl of antibody to label 106 cells in 100µl.

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Relevance

    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
  • Cellular localization

    Nuclear
  • Alternative names

    • c-myc tag antibody
    • Myc Epitope Tag antibody

References

This product has been referenced in:

  • Huang IC  et al. Distinct patterns of IFITM-mediated restriction of filoviruses, SARS coronavirus, and influenza A virus. PLoS Pathog 7:e1001258 (2011). Read more (PubMed: 21253575) »
  • Spandidos DA  et al. Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue. Anticancer Res 7:1299-304 (1987). IHC-P . Read more (PubMed: 3327455) »
See all 2 Publications for this product

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