Western blot abreview for Anti-c-Myc antibody [9E10] (HRP)

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (293T cells)
Loading amount
20 µg
Specification
293T cells
Gel Running Conditions
Reduced Denaturing (Invitrogen NUPAGE Bis-Tris Gel 4-12% Gradient Gel MES Buffer)
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/50000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST (0.2% Tween20) containing 5% milk

Secondary antibody

Secondary antibody
None used

Detection

Detection method
Pico and Femto ECL
Exposure
10 second(s)
Bands
Specific: 104 kDa
Positive control
Mouse RFX4 tagged with C-terminal myc tag in pCAG plasmid
Negative control
293T cells transfected with EGFP

Additional data

Additional Notes
I detected Mouse RFX1 tagged with C-terminal myc tag in pCAG plasmid using this antibody.
Extremely clean band. No non-specific bands. I love it.

Abcam user community

Verified customer

Submitted Mar 25 2010

Sign up