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Human Cell lysate - whole cell (293T cells)
Gel Running Conditions
Reduced Denaturing (Invitrogen NUPAGE Bis-Tris Gel 4-12% Gradient Gel MES Buffer)
Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST (0.2% Tween20) containing 5% milk
Pico and Femto ECL
Mouse RFX4 tagged with C-terminal myc tag in pCAG plasmid
293T cells transfected with EGFP
I detected Mouse RFX1 tagged with C-terminal myc tag in pCAG plasmid using this antibody.
Extremely clean band. No non-specific bands. I love it.
Abcam user community
Submitted Mar 25 2010