Western blot abreview for Anti-c-Myc antibody [9E10] (HRP)

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Abreviews
Application
Western blot
Sample
Escherichia coli Recombinant protein (BL21(DE3))
Loading amount
0.5 µg
Specification
BL21(DE3)
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Dilution
1/50000
Incubation time
1 hour(s) and 30 minute(s) · Diluent: TBST+5%Milk

Secondary antibody

Secondary antibody
None used

Detection

Detection method
ECL+
Exposure
15 second(s)
Bands
Specific: 44 kDa

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Submitted Mar 30 2010

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