Product nameAnti-Myc tag antibody [9E10] (Phycoerythrin)
See all Myc tag primary antibodies
DescriptionMouse monoclonal [9E10] to Myc tag (Phycoerythrin)
ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
Tested applicationsSuitable for: Flow Cytmore details
Synthetic peptide within Human c-Myc aa 408-432 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Conjugate = SureLight® R-Phycoerythrin.
We recommend that the investigator determine the appropriate working concentration for their specific application.
Excitation max. lambda: 565>498 nm Emission max. lambda: 578 nm F/P 1:1.
Storage instructionsShipped at 4°C. Store at +4°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
Light chain typekappa
- Epigenetics and Nuclear Signaling
- Domain Families
- HLH / Leucine Zipper
- HLH / Leucine Zipper
- Anti-Myc tag antibody [9E10] (DyLight® 650) (ab117487)
- Anti-Myc tag antibody [9E10] (DyLight® 488) (ab117499)
- Anti-Myc tag antibody [9E10] (DyLight® 550) (ab117511)
- Anti-Myc tag antibody [9E10] (FITC) (ab117599)
- Anti-Myc tag antibody [9E10] - C-terminal (Biotin) (ab197139)
- Anti-Myc tag antibody [9E10] (Alexa Fluor® 488) (ab202008)
- Anti-Myc tag antibody [9E10] (ab206486)
- Anti-Myc tag antibody [9E10] (Alexa Fluor® 594) (ab223894)
- Anti-Myc tag antibody [9E10] (Alexa Fluor® 647) (ab223895)
- Anti-Myc tag antibody [9E10] (Allophycocyanin) (ab223896)
- Anti-Myc tag antibody [9E10] - ChIP Grade (ab32)
- Anti-Myc tag antibody [9E10] (HRP) (ab62928)
- Anti-Myc tag antibody [9E10] (SureLight® Allophycocyanin) (ab72580)
- Anti-Myc tag antibody [9E10] (Biotin) (ab81658)
Our Abpromise guarantee covers the use of ab72468 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The cellular localisation of this product has been verified in ICC/IF.
ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
RelevanceEpitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
- c-myc tag antibody
- Myc Epitope Tag antibody
Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-tagged TMEM119 expression vector labelling Myc-tag with ab72468 at 1/500 dilution (right) compared with Mouse IgG1 (monoclonal) Phycoerythrin (left). Cells were surface-stained with TMEM119 antibody (ab210405), then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Phycoerythrin conjugated Myc-tag antibody (ab72468) and Alexa Fluor® 488 conjugated anti-Rabbit secondary antibody (for TMEM119). Only TMEM119 (+) population showed Myc-tag positive staining.
This product has been referenced in:
- Harwood SL et al. ATTACK, a novel bispecific T cell-recruiting antibody with trivalent EGFR binding and monovalent CD3 binding for cancer immunotherapy. Oncoimmunology 7:e1377874 (2017). WB ; Human . Read more (PubMed: 29296540) »