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    myc-tag-antibody-9e11-chip-grade-ab56.pdf

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Tags & Cell Markers Epitope Tags Myc Tag
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Anti-Myc tag antibody [9E11] - ChIP Grade (ab56)

  • Datasheet
  • SDS
Reviews (7)Q&A (5)References (62)

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Immunoprecipitation - Anti-Myc tag antibody [9E11] - ChIP Grade (ab56)
  • Western blot - Anti-Myc tag antibody [9E11] - ChIP Grade (ab56)
  • Flow Cytometry - Anti-Myc tag antibody [9E11] - ChIP Grade (ab56)
  • Western blot - Anti-Myc tag antibody [9E11] - ChIP Grade (ab56)

Key features and details

  • Mouse monoclonal [9E11] to Myc tag - ChIP Grade
  • Suitable for: Flow Cyt, ChIP, WB, IP, IHC-P, IHC-Fr
  • Reacts with: Chicken, Human, Saccharomyces cerevisiae, Recombinant fragment
  • Isotype: IgG2a

You may also be interested in

Primary
HRP Anti-Myc tag antibody (ab1326)
Protein
Recombinant human c-Myc protein (Active) (ab169901)
Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

View more associated products

Overview

  • Product name

    Anti-Myc tag antibody [9E11] - ChIP Grade
    See all Myc tag primary antibodies
  • Description

    Mouse monoclonal [9E11] to Myc tag - ChIP Grade
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, ChIP, WB, IP, IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Chicken, Human, Saccharomyces cerevisiae, Recombinant fragment
  • Immunogen

    Synthetic peptide corresponding to Human Myc tag aa 400-500 conjugated to keyhole limpet haemocyanin.

  • Positive control

    • Purchase matching WB positive control:Recombinant human c-Myc protein (Active)
    • IP:CHO overexpressing Stra8 whole cell lysate. Flow Cyt: HL60 cells. WB: ab84132, ab5395
  • General notes

    This antibody clone is manufactured by Abcam.

    ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Monoclonal
  • Clone number

    9E11
  • Myeloma

    Sp2
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • Myc Tag
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH / Leucine Zipper
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Nuclear
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cancer
    • Cell cycle
    • Cell differentiation
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors
    • Cancer
    • Tumor biomarkers
    • Oncoproteins
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP antibodies

Associated products

  • Alternative Versions

    • Anti-Myc tag antibody [9E11] - BSA and Azide free (ab264433)
  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Recombinant Protein

    • Recombinant human c-Myc protein (Active) (ab169901)
  • Related Products

    • Human c-Myc peptide (ab166837)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab56 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
1/200.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

ChIP (1)
Use at an assay dependent concentration. 2ul per 500ug of extract, see H. Zhang et al.
WB (6)
1/500 - 1/1000. Predicted molecular weight: 49 kDa. Additional non-specific bands observed at 75, 110, 140 kDa using mouse and human cells (see Abreview).
IP
Use a concentration of 5 µg/ml.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Notes
Flow Cyt
1/200.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

ChIP
Use at an assay dependent concentration. 2ul per 500ug of extract, see H. Zhang et al.
WB
1/500 - 1/1000. Predicted molecular weight: 49 kDa. Additional non-specific bands observed at 75, 110, 140 kDa using mouse and human cells (see Abreview).
IP
Use a concentration of 5 µg/ml.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

Target

  • Relevance

    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
  • Cellular localization

    Nuclear
  • Database links

    • Entrez Gene: 4609 Human
    • Omim: 190080 Human
    • SwissProt: P01106 Human
    • Unigene: 202453 Human
    • Alternative names

      • c-myc tag antibody
      • Myc Epitope Tag antibody

    Images

    • Immunoprecipitation - Anti-Myc tag antibody [9E11] - ChIP Grade (ab56)
      Immunoprecipitation - Anti-Myc tag antibody [9E11] - ChIP Grade (ab56)

      Myc tag was immunoprecipitated using 0.5mg CHO overexpressing Stra8 whole cell lysate, 5µg of Mouse monoclonal to Myc tag and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

      The antibody was incubated under agitation with Protein G beads for 10min, CHO overexpressing Stra8 whole cell lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

      Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab56.

      Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.

      Band: 49KDa; Myc tag

    • Western blot - Anti-Myc tag antibody [9E11] - ChIP Grade (ab56)
      Western blot - Anti-Myc tag antibody [9E11] - ChIP Grade (ab56)
      Anti-Myc tag antibody [9E11] - ChIP Grade (ab56) at 1/500 dilution + Recombinant Human c-Myc protein (ab84132) at 0.01 µg

      Secondary
      Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 49 kDa


      Exposure time: 4 minutes
    • Flow Cytometry - Anti-Myc tag antibody [9E11] - ChIP Grade (ab56)
      Flow Cytometry - Anti-Myc tag antibody [9E11] - ChIP Grade (ab56)

      Overlay histogram showing HL60 cells stained with ab56 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    • Western blot - Anti-Myc tag antibody [9E11] - ChIP Grade (ab56)
      Western blot - Anti-Myc tag antibody [9E11] - ChIP Grade (ab56)
      All lanes : Anti-Myc tag antibody [9E11] - ChIP Grade (ab56) at 1 µg/ml

      Lane 1 : HEK293 cell lysate at 20 µg
      Lane 2 : Recombinant Human c-Myc protein (ab84132) at 0.1 µg
      Lane 3 : E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.1 µg

      Secondary
      All lanes : Goat polyclonal to Mouse IgG H&L Pre-Adsorbed (HRP) at 1/5000 dilution

      Predicted band size: 49 kDa

    Protocols

    • ChIP protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (62)

    Publishing research using ab56? Please let us know so that we can cite the reference in this datasheet.

    ab56 has been referenced in 62 publications.

    • Zhao W  et al. Splicing factor derived circular RNA circUHRF1 accelerates oral squamous cell carcinoma tumorigenesis via feedback loop. Cell Death Differ 27:919-933 (2020). PubMed: 31570856
    • Liu M  et al. PRMT5-dependent transcriptional repression of c-Myc target genes promotes gastric cancer progression. Theranostics 10:4437-4452 (2020). PubMed: 32292506
    • Yu H  et al. LOXL1 confers antiapoptosis and promotes gliomagenesis through stabilizing BAG2. Cell Death Differ N/A:N/A (2020). PubMed: 32424143
    • Fan L  et al. Histone demethylase JMJD1A promotes expression of DNA repair factors and radio-resistance of prostate cancer cells. Cell Death Dis 11:214 (2020). PubMed: 32238799
    • Yasumizu Y  et al. MUC1-C regulates lineage plasticity driving progression to neuroendocrine prostate cancer. Nat Commun 11:338 (2020). PubMed: 31953400
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 12 Abreviews or Q&A

    Western blot abreview for Anti-c-Myc antibody [9E11] - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing (4-12% Nupage gel)
    Sample
    Human Cell lysate - whole cell (HEK 293)
    Specification
    HEK 293
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 26 2014

    Western blot abreview for Anti-c-Myc antibody [9E11] - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MCF7)
    Loading amount
    10 µg
    Specification
    MCF7
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 26 2011

    ChIP abreview for Anti-c-Myc antibody [9E11] - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - whole cell (skin fibroblasts)
    Specification
    skin fibroblasts
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: Formaldehyde
    Detection step
    Real-time PCR
    Positive control
    NPM1 cMYC binding site
    Negative control
    IgG pull-down
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 15 2011

    Western blot abreview for Anti-c-Myc antibody [9E11] - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (huh-7)
    Loading amount
    15 µg
    Specification
    huh-7
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 08 2010

    Western blot abreview for Anti-c-Myc antibody [9E11] - ChIP Grade

    Average
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (mammary epithelial)
    Loading amount
    80 µg
    Specification
    mammary epithelial
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 25 2009

    Western blot abreview for Anti-c-Myc antibody [9E11] - ChIP Grade

    Below Average
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Breast cancer cell-lines)
    Loading amount
    20 µg
    Specification
    Breast cancer cell-lines
    Gel Running Conditions
    Non-reduced Denaturing (4-20% Nupage gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. Cherie Blenkiron

    Verified customer

    Submitted Jul 18 2008

    Question

    I'd like to know whether this antibody works efficiently in ChIP (conventional paraformaldehyde fixation) of Myc-tagged (one tag) proteins expressed in mammalian tissue culture cells. .

    Read More

    Abcam community

    Verified customer

    Asked on Jan 22 2007

    Answer

    This antibody was qualified as "ChIP grade" following its application in the following publications; Robert F et al. Global position and recruitment of HATs and HDACs in the yeast genome. Mol Cell 16:199-209 (2004). PubMed: 15494307 Zhang H et al. The Yaf9 component of the SWR1 and NuA4 complexes is required for proper gene expression, histone H4 acetylation, and Htz1 replacement near telomeres. Mol Cell Biol 24:9424-36 (2004). PubMed: 15485911 We have received reports suggesting that the antibody does not give a huge enrichment but that the enrichment is specific. Furthermore it is guaranteed for this purpose. I would recommend that you consult the publications for details of the approach that was adopted. From my recollection they did use proteins tagged with more than one Myc-tag.

    Read More

    Abcam Scientific Support

    Answered on Jan 23 2007

    Western blot abreview for Anti-c-Myc antibody [9E11] - ChIP Grade

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Pancreatic cell lines)
    Specification
    Pancreatic cell lines
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Dr. Lynda Elghazi-Cras

    Verified customer

    Submitted Jul 25 2005

    Question

    I would like to know which of your human breast tumor slides would be the best positive control for antibody ab56.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 11 2004

    Answer

    Thank you for your enquiry and your interest in our products. I would suggest taking a look at the published references to have some more information about the breast cancer cell types in which c-myc is expressed. Locker AP et al. c-myc oncogene product expression and prognosis in operable breast cancer. Br J Cancer 60:669-72 (1989). PubMed: 2679850 Once you know which tumour types you are looking for, you can conduct a search in our on-line catalogue. We have several human breast tissue slides such as: ab4696: fibroadenoma, ab4701: adenocarcinoma, ab4697: dusctal carcinoma, ab4698: lobular carcinoma, ab4700: medullary carcinoma

    Read More

    Abcam Scientific Support

    Answered on Mar 12 2004

    Question

    Can I use c-myc 9e11 for histochemical localization in paraffin embedded sections and what antigen retrival steps do I take and what conc is optimum??

    Read More

    Abcam community

    Verified customer

    Asked on Aug 06 2001

    Answer

    Thsi antibody works well for histochemical localization in paraffin embedded sections. We would recommend a microwave retrieval (10 minutes in 0.01M citrate buffer, pH 6.0, the specific time will depend on the power of the microwave). Leave the 1ary antibody on overnight at a concentration of 5ug/ml.

    Read More

    Abcam Scientific Support

    Answered on Aug 06 2001

    1-10 of 12 Abreviews or Q&A

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