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I have tested this antibody again in a cell line expressing a myc-tagged protein, well recognized by 9E10. It again did not work for me. Do you have any suggestions (I filled out the technical inquiry form at length).
Asked on Mar 23 2004
Thank you for your patience. I was away on vacation and could not get back to you earlier. We have had no other complaints regarding this antibody. You're getting absolutely no signal? Are you doing antigen retrieval? You stated that your cells are fixed with 4% paraformaldehyde and that may be modifying the epitope that the antibody recognizes. At this point that is my suggestion as well as trying a higher concentration of the antibody. Let me know what you think.
Answered on Mar 26 2004