Western blot abreview for Anti-Myc tag antibody

Average
Abreviews
Application
Western blot
Loading amount
35 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - whole (Liver)
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Incubation time
16 hour(s) and 0 minute(s)
Dilution
1/2000

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti-chicken HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
ECL
Bands
Specific: 47 kDa Non-specific: 55 kb kDa
Exposure
1 minute(s) and 0 second(s)
Negative control
WT mice
Positive control
Samples from mice with a myc-tagged gene

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Verified customer

Submitted May 16 2014

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